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Galectin 1/LGALS1 polyclonal antibody

货号: BS70195

价格：￥ 1140

货期：现货（1-2个工作日）

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说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :BS70195

Product Name :Galectin 1/LGALS1 polyclonal antibody

Swiss-Prot :P09382

Host :Rabbit

Reactivity :Human, Mouse, Rat

Applications :WB, IF/ICC

Application_all :WB,1:1000 - 1:4000|IF/ICC,1:50 - 1:200

Background :The galectins are a family of beta-galactoside-binding proteins implicated in modulating cell-cell and cell-matrix interactions. This gene product may act as an autocrine negative growth factor that regulates cell proliferation.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Polyclonal Antibodies

BiowMW :14KDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :LGALS1;GAL1;GBP

Immunogen :Recombinant fusion protein of human Galectin 1/Galectin 1/LGALS1(NP_002296.1).

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of extracts of various cell lines, using Galectin 1/LGALS1 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 30s.

Immunofluorescence analysis of NIH/3T3 cells using Galectin 1/LGALS1 Rabbit pAb at dilution of 1:50 . Blue: DAPI for nuclear staining.

Immunofluorescence analysis of PC-12 cells using Galectin 1/LGALS1 Rabbit pAb at dilution of 1:50 . Blue: DAPI for nuclear staining.

Immunofluorescence analysis of U2OS cells using Galectin 1/LGALS1 Rabbit pAb at dilution of 1:50 . Blue: DAPI for nuclear staining.