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Transferrin polyclonal antibody

货号: BS70160

价格：￥ 1140

货期：现货（1-2个工作日）

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No. :BS70160

Product Name :Transferrin polyclonal antibody

Swiss-Prot :P02787

Host :Rabbit

Reactivity :Human, Mouse, Rat

Applications :WB,IF/ICC

Application_all :WB,1:500 - 1:2000|IF/ICC,1:50 - 1:200

Background :This gene encodes a glycoprotein with an approximate molecular weight of 76.5 kDa. It is thought to have been created as a result of an ancient gene duplication event that led to generation of homologous C and N-terminal domains each of which binds one ion of ferric iron. The function of this protein is to transport iron from the intestine, reticuloendothelial system, and liver parenchymal cells to all proliferating cells in the body. This protein may also have a physiologic role as granulocyte/pollen-binding protein (GPBP) involved in the removal of certain organic matter and allergens from serum.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Polyclonal Antibodies

BiowMW :77KDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :HEL-S-71p;PRO1557;PRO2086;TFQTL1;TF;Transferrin

Immunogen :Recombinant fusion protein of human Transferrin(NP_001054.2).

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of extracts of various cell lines, using Transferrin antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 1s.

Immunofluorescence analysis of HepG2 cells using Transferrin Rabbit pAb at dilution of 1:50 . Blue: DAPI for nuclear staining.

Immunofluorescence analysis of human liver cells using Transferrin Rabbit pAb at dilution of 1:50 . Blue: DAPI for nuclear staining.

Cholesterol sequestration by nystatin enhances the uptake and activity of endostatin in endothelium via regulating distinct endocytic pathways

JournalBlood

ApplicationWB

IF16.562

SpeciesHuman

PMID21482707