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COL2A1 polyclonal antibody

货号: BS70156

价格：￥ 1140

货期：现货（1-2个工作日）

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No. :BS70156

Product Name :COL2A1 polyclonal antibody

Swiss-Prot :P02458

Host :Rabbit

Reactivity :Human, Mouse

Applications :WB, IF/ICC

Application_all :WB,1:500 - 1:2000|IF/ICC,1:50 - 1:200

Background :This gene encodes the alpha-1 chain of type II collagen, a fibrillar collagen found in cartilage and the vitreous humor of the eye. Mutations in this gene are associated with achondrogenesis, chondrodysplasia, early onset familial osteoarthritis, SED congenita, Langer-Saldino achondrogenesis, Kniest dysplasia, Stickler syndrome type I, and spondyloepimetaphyseal dysplasia Strudwick type. In addition, defects in processing chondrocalcin, a calcium binding protein that is the C-propeptide of this collagen molecule, are also associated with chondrodysplasia. There are two transcripts identified for this gene.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Polyclonal Antibodies

BiowMW :141KDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :COL2A1;ANFH;AOM;COL11A3;SEDC;STL1

Immunogen :A synthetic peptide of human COL2A1(NP_001835.3).

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of Mouse skeletal muscle, using COL2A1 antibody at 1:1640 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 10s.

Immunofluorescence analysis of HepG2 cells using COL2A1 Rabbit pAb at dilution of 1:150 . Blue: DAPI for nuclear staining.