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Alpha-1 Antitrypsin (SERPINA1) polyclonal antibody

货号: BS6957

价格：￥ 1140

货期：现货（1-2个工作日）

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说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :BS6957

Product Name :Alpha-1 Antitrypsin (SERPINA1) polyclonal antibody

Swiss-Prot :P01009

Host :Rabbit

Reactivity :Human, Mouse, Rat

Applications :WB, IHC, IF/ICC

Application_all :WB,1:500 - 1:2000|IHC,1:50 - 1:200|IF/ICC,1:50 - 1:200

Background :The protein encoded by this gene is secreted and is a serine protease inhibitor whose targets include elastase, plasmin, thrombin, trypsin, chymotrypsin, and plasminogen activator. Defects in this gene can cause emphysema or liver disease. Several transcript variants encoding the same protein have been found for this gene.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Polyclonal Antibodies

BiowMW :51KDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :SERPINA1;A1A;A1AT;AAT;PI;PI1;PRO2275;alpha1AT

Immunogen :Recombinant fusion protein of human Alpha-1 Antitrypsin (Alpha-1 Antitrypsin (SERPINA1))(NP_000286.3).

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of extracts of various cell lines, using Alpha-1 Antitrypsin antibody at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 60s.

Immunohistochemistry of paraffin-embedded mouse kidney using Alpha-1 Antitrypsin ) Rabbit pAb at dilution of 1:100 .Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Immunofluorescence analysis of HepG2 cells using Alpha-1 Antitrypsin Rabbit pAb at dilution of 1:50 . Blue: DAPI for nuclear staining.