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Presenilin 1 polyclonal antibody

货号: BS6929

价格：￥ 1140

货期：现货（1-2个工作日）

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No. :BS6929

Product Name :Presenilin 1 polyclonal antibody

Swiss-Prot :P49768

Host :Rabbit

Reactivity :Human, Mouse, Rat

Applications :WB, IHC, IF/ICC

Application_all :WB,1:500 - 1:2000|IHC,1:50 - 1:200|IF/ICC,1:50 - 1:200

Background :Alzheimer's disease (AD) patients with an inherited form of the disease carry mutations in the presenilin proteins (PSEN1; PSEN2) or in the amyloid precursor protein (APP). These disease-linked mutations result in increased production of the longer form of amyloid-beta (main component of amyloid deposits found in AD brains). Presenilins are postulated to regulate APP processing through their effects on gamma-secretase, an enzyme that cleaves APP. Also, it is thought that the presenilins are involved in the cleavage of the Notch receptor, such that they either directly regulate gamma-secretase activity or themselves are protease enzymes. Several alternatively spliced transcript variants encoding different isoforms have been identified for this gene, the full-length nature of only some have been determined.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Polyclonal Antibodies

BiowMW :52kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :AD3;FAD;PS-1;PS1;S182;Presenilin 1;PSEN1

Immunogen :Recombinant fusion protein of human Presenilin 1(NP_000012.1).

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of extracts of U-87MG cells, using Presenilin 1 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit .
Exposure time: 30s.

Immunohistochemistry of paraffin-embedded human liver using Presenilin 1 Rabbit pAb at dilution of 1:100 .Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Immunofluorescence analysis of C6 cells using Presenilin 1 Rabbit pAb at dilution of 1:100 . Blue: DAPI for nuclear staining.

Immunofluorescence analysis of HeLa cells using Presenilin 1 Rabbit pAb at dilution of 1:100 . Blue: DAPI for nuclear staining.

Tangeretin enhances radiosensitivity and inhibits the radiation-induced epithelial-mesenchymal transition of gastric cancer cells

JournalOncology Reports

ApplicationWB

IF3.906

SpeciesHuman

PMID25998143