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[KO Validated] IDH1 polyclonal antibody

货号: BS6918

价格：￥ 1140

货期：现货（1-2个工作日）

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No. :BS6918

Product Name :[KO Validated] IDH1 polyclonal antibody

Swiss-Prot :O75874

Host :Rabbit

Reactivity :Human, Mouse, Rat

Applications :WB, IF/ICC, IP

Application_all :WB,1:500 - 1:2000|IF/ICC,1:50 - 1:200|IP,1:50 - 1:100

Background :Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. Each NADP(+)-dependent isozyme is a homodimer. The protein encoded by this gene is the NADP(+)-dependent isocitrate dehydrogenase found in the cytoplasm and peroxisomes. It contains the PTS-1 peroxisomal targeting signal sequence. The presence of this enzyme in peroxisomes suggests roles in the regeneration of NADPH for intraperoxisomal reductions, such as the conversion of 2, 4-dienoyl-CoAs to 3-enoyl-CoAs, as well as in peroxisomal reactions that consume 2-oxoglutarate, namely the alpha-hydroxylation of phytanic acid. The cytoplasmic enzyme serves a significant role in cytoplasmic NADPH production. Alternatively spliced transcript variants encoding the same protein have been found for this gene.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Polyclonal Antibodies

BiowMW :46KDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :IDH1;HEL-216;HEL-S-26;IDCD;IDH;IDP;IDPC;PICD

Immunogen :Recombinant fusion protein of human IDH1(NP_005887.2).

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of extracts from normal and IDH1 knockout 293T cells, using IDH1 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 60s.

Immunofluorescence analysis of C6 cells using IDH1 Polyclonal Antibody at dilution of 1:100 . Blue: DAPI for nuclear staining.

Immunofluorescence analysis of L929 cells using IDH1 Polyclonal Antibody at dilution of 1:100 . Blue: DAPI for nuclear staining.

Immunoprecipitation analysis of 200ug extracts of HeLa cells, using 3 ug IDH1 antibody . Western blot was performed from the immunoprecipitate using IDH1 antibody at a dilution of 1:1000.