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HLA-DQA1 polyclonal antibody

货号: BS6917
价格 1140
货期 现货(1-2个工作日)
25ul 50ul 100ul 200ul
No. :BS6917
Product Name :HLA-DQA1 polyclonal antibody
Swiss-Prot :P01909
Host :Rabbit
Reactivity :Human, Mouse
Applications :WB, IHC
Application_all :WB,1:500 - 1:2000|IHC,1:50 - 1:200
Background :HLA-DQA1 belongs to the HLA class II alpha chain paralogues. The class II molecule is a heterodimer consisting of an alpha (DQA) and a beta chain (DQB), both anchored in the membrane. It plays a central role in the immune system by presenting peptides derived from extracellular proteins. Class II molecules are expressed in antigen presenting cells (APC: B Lymphocytes, dendritic cells, macrophages). The alpha chain is approximately 33-35 kDa. It is encoded by 5 exons; exon 1 encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, and exon 4 encodes the transmembrane domain and the cytoplasmic tail. Within the DQ molecule both the alpha chain and the beta chain contain the polymorphisms specifying the peptide binding specificities, resulting in up to four different molecules. Typing for these polymorphisms is routinely done for bone marrow transplantation.
Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity :Polyclonal Antibodies
BiowMW :28kDaa-37kDa
Note :For research use only, not for use in diagnostic procedure.
Alternative Name :HLA-DQA1;CELIAC1;DQ-A1;HLA-DQA;CD;GSE;class II
Immunogen :Recombinant fusion protein of human HLA-DQA1(NP_002113.2).
Conjugate :Unconjugated
Modification :Unmodification
Western blot analysis of extracts of various cell lines, using HLA-DQA1 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Immunohistochemistry of paraffin-embedded human prostate using HLA-DQA1 antibody at dilution of 1:100 .Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunohistochemistry of paraffin-embedded human esophagus using HLA-DQA1 antibody at dilution of 1:100 .Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

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400-660-9091

电话:025-68037686

地址:江苏生命科技创新园F6幢1层

订购:nanjing03@biogot.com

服务:biorase01@biogot.com

合作:lvyun@biogot.com

支持:may@biogot.com