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[KO Validated] BRG1/SMARCA4 polyclonal antibody

货号: BS6902
价格 1140
货期 现货(1-2个工作日)
25ul 50ul 100ul 200ul
No. :BS6902
Product Name :[KO Validated] BRG1/SMARCA4 polyclonal antibody
Swiss-Prot :P51532
Host :Rabbit
Reactivity :Human, Mouse, Rat
Applications :WB, IHC, IF/ICC, IP
Application_all :WB,1:500 - 1:2000|IHC,1:50 - 1:200|IF/ICC,1:50 - 1:200|IP,1:50 - 1:200
Background :The protein encoded by this gene is a member of the SWI/SNF family of proteins and is similar to the brahma protein of Drosophila. Members of this family have helicase and ATPase activities and are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The encoded protein is part of the large ATP-dependent chromatin remodeling complex SNF/SWI, which is required for transcriptional activation of genes normally repressed by chromatin. In addition, this protein can bind BRCA1, as well as regulate the expression of the tumorigenic protein CD44. Mutations in this gene cause rhabdoid tumor predisposition syndrome type 2. Multiple transcript variants encoding different isoforms have been found for this gene.
Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity :Polyclonal Antibodies
BiowMW :220KDa
Note :For research use only, not for use in diagnostic procedure.
Alternative Name :BAF190;BAF190A;BRG1;CSS4;MRD16;RTPS2;SNF2;SNF2L4;SNF2LB;SWI2;hSNF2b;SMARCA4
Immunogen :Recombinant fusion protein of human BRG1/SMARCA4(NP_003063.2).
Conjugate :Unconjugated
Modification :Unmodification
Western blot analysis of extracts from normal and BRG1/BRG1/SMARCA4 knockout 293T cells, using BRG1/BRG1/SMARCA4 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit .
Exposure time: 1s.
Immunohistochemistry of paraffin-embedded human liver cancer using [KO Validated] BRG1/BRG1/SMARCA4 Rabbit pAb at dilution of 1:100 .Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry of paraffin-embedded mouse lung using [KO Validated] BRG1/BRG1/SMARCA4 Rabbit pAb at dilution of 1:100 .Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunofluorescence analysis of A549 cells using BRG1/BRG1/SMARCA4 antibody . Blue: DAPI for nuclear staining.

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400-660-9091

电话:025-68037686

地址:江苏生命科技创新园F6幢1层

订购:nanjing03@biogot.com

服务:biorase01@biogot.com

合作:lvyun@biogot.com

支持:may@biogot.com