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FAK polyclonal antibody

货号: BS6899
价格 1140
货期 现货(1-2个工作日)
25ul 50ul 100ul 200ul
No. :BS6899
Product Name :FAK polyclonal antibody
Swiss-Prot :Q05397
Host :Rabbit
Reactivity :Human, Mouse, Rat
Applications :WB, IHC, IF/ICC
Application_all :WB,1:1000 - 1:2000|IHC,1:50 - 1:100|IF/ICC,1:50 - 1:200
Background :This gene encodes a cytoplasmic protein tyrosine kinase which is found concentrated in the focal adhesions that form between cells growing in the presence of extracellular matrix constituents. The encoded protein is a member of the FAK subfamily of protein tyrosine kinases but lacks significant sequence similarity to kinases from other subfamilies. Activation of this gene may be an important early step in cell growth and intracellular signal transduction pathways triggered in response to certain neural peptides or to cell interactions with the extracellular matrix. Several transcript variants encoding different isoforms have been found for this gene.
Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity :Polyclonal Antibodies
BiowMW :125KDa
Note :For research use only, not for use in diagnostic procedure.
Alternative Name :FADK;FAK;FAK1;FRNK;PPP1R71;p125FAK;pp125FAK;PTK2
Immunogen :A synthetic peptide of human FAK(NP_722560.1).
Conjugate :Unconjugated
Modification :Unmodification
Western blot analysis of extracts of U-87MG cells, using FAK antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 10s.
Immunohistochemistry of paraffin-embedded human esophagus using FAK antibody at dilution of 1:100 .Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunohistochemistry of paraffin-embedded rat kidney using FAK antibody at dilution of 1:100 .Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunofluorescence analysis of C6 cells using FAK Rabbit pAb at dilution of 1:100 . Blue: DAPI for nuclear staining.
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400-660-9091

电话:025-68037686

地址:江苏生命科技创新园F6幢1层

订购:nanjing03@biogot.com

服务:biorase01@biogot.com

合作:lvyun@biogot.com

支持:may@biogot.com