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Integrin alpha V (ITGAV/CD51) polyclonal antibody

货号: BS6891
价格 1140
货期 现货(1-2个工作日)
25ul 50ul 100ul 200ul
No. :BS6891
Product Name :Integrin alpha V (ITGAV/CD51) polyclonal antibody
Swiss-Prot :P06756
Host :Rabbit
Reactivity :Human, Mouse, Rat
Applications :WB, IHC, IP
Application_all :WB,1:500 - 1:2000|IHC,1:50 - 1:200|IP,1:50 - 1:200
Background :The product of this gene belongs to the integrin alpha chain family. Integrins are heterodimeric integral membrane proteins composed of an alpha subunit and a beta subunit that function in cell surface adhesion and signaling. The encoded preproprotein is proteolytically processed to generate light and heavy chains that comprise the alpha V subunit. This subunit associates with beta 1, beta 3, beta 5, beta 6 and beta 8 subunits. The heterodimer consisting of alpha V and beta 3 subunits is also known as the vitronectin receptor. This integrin may regulate angiogenesis and cancer progression. Alternative splicing results in multiple transcript variants. Note that the integrin alpha 5 and integrin alpha V subunits are encoded by distinct genes.
Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity :Polyclonal Antibodies
BiowMW :130KDa
Note :For research use only, not for use in diagnostic procedure.
Alternative Name :CD51;MSK8;VNRA;VTNR;Integrin alpha V;ITGAV
Immunogen :A synthetic peptide of human alpha V (ITGAV/CD51)(NP_002201.1).
Conjugate :Unconjugated
Modification :Unmodification
Western blot analysis of extracts of various cell lines, using Integrin alpha V antibody at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 3s.
Western blot analysis of extracts of MCF7 cells, using Integrin alpha V antibody at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 10s.
Immunohistochemistry of paraffin-embedded rat testis using Integrin alpha V Rabbit pAb at dilution of 1:100 .Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunoprecipitation analysis of 200ug extracts of C6 cells using 3ug Integrin alpha V antibody . Western blot was performed from the immunoprecipitate using Integrin alpha V antibody at a dilution of 1:2000.

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400-660-9091

电话:025-68037686

地址:江苏生命科技创新园F6幢1层

订购:nanjing03@biogot.com

服务:biorase01@biogot.com

合作:lvyun@biogot.com

支持:may@biogot.com