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Insulin polyclonal antibody

货号: BS6890
价格 1140
货期 现货(1-2个工作日)
25ul 50ul 100ul 200ul
No. :BS6890
Product Name :Insulin polyclonal antibody
Swiss-Prot :P01308
Host :Rabbit
Reactivity :Human, Mouse, Rat
Applications :WB, IHC, IF/ICC
Application_all :WB,1:500 - 1:2000|IHC,1:50 - 1:200|IF/ICC,1:50 - 1:200
Background :After removal of the precursor signal peptide, proinsulin is post-translationally cleaved into three peptides: the B chain and A chain peptides, which are covalently linked via two disulfide bonds to form insulin, and C-peptide. Binding of insulin to the insulin receptor (INSR) stimulates glucose uptake. A multitude of mutant alleles with phenotypic effects have been identified. There is a read-through gene, INS-IGF2, which overlaps with this gene at the 5' region and with the IGF2 gene at the 3' region. Alternative splicing results in multiple transcript variants.
Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity :Polyclonal Antibodies
BiowMW :6KDa
Note :For research use only, not for use in diagnostic procedure.
Alternative Name :IDDM;IDDM1;IDDM2;ILPR;IRDN;MODY10;INS;Insulin;insulin
Immunogen :Recombinant fusion protein of human INS(NP_000198.1).
Conjugate :Unconjugated
Modification :Unmodification
Western blot analysis of various lysates, using Insulin antibody at 1:671 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 30s.
Immunohistochemistry of paraffin-embedded rat pancreatic islet using Insulin Rabbit pAb at dilution of 1:100 .Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunofluorescence analysis of human pancreas cells using Insulin Rabbit pAb at dilution of 1:50 . Blue: DAPI for nuclear staining.
Immunofluorescence analysis of mouse pancreas cells using Insulin Rabbit pAb at dilution of 1:50 . Blue: DAPI for nuclear staining.

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400-660-9091

电话:025-68037686

地址:江苏生命科技创新园F6幢1层

订购:nanjing03@biogot.com

服务:biorase01@biogot.com

合作:lvyun@biogot.com

支持:may@biogot.com