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HDAC2 polyclonal antibody

货号: BS6887

价格：￥ 1140

货期：现货（1-2个工作日）

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No. :BS6887

Product Name :HDAC2 polyclonal antibody

Swiss-Prot :Q92769

Host :Rabbit

Reactivity :Human, Mouse, Rat

Applications :WB, IHC, IP

Application_all :WB,1:500 - 1:2000|IHC,1:50 - 1:200|IP,1:50 - 1:200

Background :This gene product belongs to the histone deacetylase family. Histone deacetylases act via the formation of large multiprotein complexes, and are responsible for the deacetylation of lysine residues at the N-terminal regions of core histones (H2A, H2B, H3 and H4). This protein forms transcriptional repressor complexes by associating with many different proteins, including YY1, a mammalian zinc-finger transcription factor. Thus, it plays an important role in transcriptional regulation, cell cycle progression and developmental events. Alternative splicing results in multiple transcript variants.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Polyclonal Antibodies

BiowMW :60kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :HD2;RPD3;YAF1;HDAC2

Immunogen :A synthetic peptide of human HDAC2(NP_001518.3).

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of extracts of various cell lines, using HDAC2 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

Immunohistochemistry of paraffin-embedded rat kidney using HDAC2 antibody at dilution of 1:100 .Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Immunohistochemistry of paraffin-embedded human esophageal cancer using HDAC2 antibody at dilution of 1:100 .Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Immunohistochemistry of paraffin-embedded mouse heart using HDAC2 antibody at dilution of 1:100 .Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

p53 suppresses lung resistance-related protein expression through Y-box binding protein 1 in the MCF-7 breast tumor cell line

JournalJournal Of Cellular Physiology

ApplicationWB

IF6.384

SpeciesHuman

PMID
Expression patterns of histone deacetylases in experimental stroke and potential targets for neuroprotection

JournalClinical And Experimental Pharmacology And Physiology

ApplicationWB

IF2.336

SpeciesMouse

PMID22651689
The subcellular distribution and function of MTA1 in cancer differentiation

JournalOncotarget

ApplicationWB,IP

IF0

SpeciesHuman

PMID24970816
The long non-coding RNA TUG1 indicates a poor prognosis for colorectal cancer and promotes metastasis by affecting epithelial-mesenchymal transition

JournalJournal of Translational Medicine

ApplicationWB

IF4.098

SpeciesHuman

PMID26856330