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Androgen Receptor polyclonal antibody

货号: BS6872

价格：￥ 1140

货期：现货（1-2个工作日）

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说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :BS6872

Product Name :Androgen Receptor polyclonal antibody

Swiss-Prot :P10275

Host :Rabbit

Reactivity :Human, Mouse, Rat

Applications :WB

Application_all :WB,1:500 - 1:2000

Background :The androgen receptor gene is more than 90 kb long and codes for a protein that has 3 major functional domains: the N-terminal domain, DNA-binding domain, and androgen-binding domain. The protein functions as a steroid-hormone activated transcription factor. Upon binding the hormone ligand, the receptor dissociates from accessory proteins, translocates into the nucleus, dimerizes, and then stimulates transcription of androgen responsive genes. This gene contains 2 polymorphic trinucleotide repeat segments that encode polyglutamine and polyglycine tracts in the N-terminal transactivation domain of its protein. Expansion of the polyglutamine tract from the normal 9-34 repeats to the pathogenic 38-62 repeats causes spinal bulbar muscular atrophy (Kennedy disease). Mutations in this gene are also associated with complete androgen insensitivity (CAIS). Two alternatively spliced variants encoding distinct isoforms have been described.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Polyclonal Antibodies

BiowMW :80KDa/110KDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :AIS;AR8;DHTR;HUMARA;HYSP1;KD;NR3C4;SBMA;SMAX1;TFM;AR

Immunogen :A synthetic peptide of human Androgen Receptor(NP_000035.2).

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of extracts of Rat testis, using Androgen Receptor antibody at 1:668 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 3s.

Western blot analysis of extracts of Mouse testis, using Androgen Receptor antibody at 1:668 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 10s.

Immunofluorescence analysis of MCF7 cells using Androgen Receptor Rabbit pAb at dilution of 1:100 . Blue: DAPI for nuclear staining.

Polyol Pathway Mediates Enhanced Degradation of Extracellular Matrix Via p38 MAPK Activation in Intervertebral Disc of Diabetic Rats

JournalConnective Tissue Research

ApplicationWB

IF2.795

SpeciesRat

PMID23215968