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PLCB1 polyclonal antibody

货号: BS6868

价格：￥ 1140

货期：现货（1-2个工作日）

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No. :BS6868

Product Name :PLCB1 polyclonal antibody

Swiss-Prot :Q9NQ66

Host :Rabbit

Reactivity :Human, Mouse, Rat

Applications :WB, IHC

Application_all :WB,1:500 - 1:2000|IHC,1:50 - 1:200

Background :The protein encoded by this gene catalyzes the formation of inositol 1,4,5-trisphosphate and diacylglycerol from phosphatidylinositol 4,5-bisphosphate. This reaction uses calcium as a cofactor and plays an important role in the intracellular transduction of many extracellular signals. This gene is activated by two G-protein alpha subunits, alpha-q and alpha-11. Two transcript variants encoding different isoforms have been found for this gene.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Polyclonal Antibodies

BiowMW :139kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :PLCB1;EIEE12;PI-PLC;PLC-154;PLC-I;PLC-beta-1;PLC154;PLCB1A;PLCB1B

Immunogen :Recombinant fusion protein of human PLCB1(NP_056007.1).

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of extracts of NCI-H460 cells, using PLCB1 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

Immunohistochemistry of paraffin-embedded rat brain using PLCB1 antibody at dilution of 1:100 .Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.