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Bad polyclonal antibody

货号: BS6845

价格：￥ 1140

货期：现货（1-2个工作日）

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说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :BS6845

Product Name :Bad polyclonal antibody

Swiss-Prot :Q92934

Host :Rabbit

Reactivity :Human, Mouse, Rat

Applications :WB, IHC, IF/ICC

Application_all :WB,1:500 - 1:2000|IHC,1:50 - 1:200|IF/ICC,1:10 - 1:100

Background :The protein encoded by this gene is a member of the BCL-2 family. BCL-2 family members are known to be regulators of programmed cell death. This protein positively regulates cell apoptosis by forming heterodimers with BCL-xL and BCL-2, and reversing their death repressor activity. Proapoptotic activity of this protein is regulated through its phosphorylation. Protein kinases AKT and MAP kinase, as well as protein phosphatase calcineurin were found to be involved in the regulation of this protein. Alternative splicing of this gene results in two transcript variants which encode the same isoform.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Polyclonal Antibodies

BiowMW :15kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :BAD;BBC2;BCL2L8;Bad

Immunogen :A synthetic peptide of human Bad(NP_004313.1).

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of extracts of various cell lines, using Bad antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 90s.

Immunohistochemistry of paraffin-embedded rat kidney using Bad antibody at dilution of 1:200 .Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Immunofluorescence analysis of HeLa cells using Bad antibody . Blue: DAPI for nuclear staining.

Microcystin-LR (MCLR) Induces a Compensation of PP2A Activity Mediated by α4 Protein in HEK293 Cells

JournalInternational Journal of Biological Sciences

ApplicationWB

IF4.858

SpeciesHuman

PMID21698000
Effects of PEG-liposomal oxaliplatin on apoptosis, and expression of Cyclin A and Cyclin D1 in colorectal cancer cells

JournalOncology Reports

ApplicationWB

IF3.906

SpeciesHuman

PMID22710431
Tetrandrine Induces Mitochondria-Mediated Apoptosis in Human Gastric Cancer BGC-823 Cells

JournalPLoS One

ApplicationWB

IF3.24

SpeciesHuman

PMID24098511
Silencing Prion Protein in MDA-MB-435 Breast Cancer Cells Leads to Pleiotropic Cellular Responses to Cytotoxic Stimuli

JournalPLoS One

ApplicationWB

IF2.776

SpeciesHuman

PMID23133614
Resveratrol inhibits Extranodal NK/T cell lymphoma through activation of DNA damage response pathway

JournalJournal Of Experimental & Clinical Cancer Research

ApplicationWB

IF5.646

SpeciesHuman

PMID28950914