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[KO Validated] NADPH oxidase 4 (NOX4) polyclonal antibody

货号: BS6796

价格：￥ 1140

货期：现货（1-2个工作日）

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详细信息检测数据实验指南文献

No. :BS6796

Product Name :[KO Validated] NADPH oxidase 4 (NOX4) polyclonal antibody

Swiss-Prot :Q9NPH5

Host :Rabbit

Reactivity :Human, Mouse, Rat

Applications :WB, IHC

Application_all :WB,1:500 - 1:2000|IHC,1:50 - 1:200

Background :This gene encodes a member of the NOX-family of enzymes that functions as the catalytic subunit the NADPH oxidase complex. The encoded protein is localized to non-phagocytic cells where it acts as an oxygen sensor and catalyzes the reduction of molecular oxygen to various reactive oxygen species (ROS). The ROS generated by this protein have been implicated in numerous biological functions including signal transduction, cell differentiation and tumor cell growth. A pseudogene has been identified on the other arm of chromosome 11. Alternative splicing results in multiple transcript variants.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Polyclonal Antibodies

BiowMW :65KDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :NOX4;KOX;KOX-1;RENOX

Immunogen :A synthetic peptide of human NADPH oxidase 4 (NOX4)(NP_058627.2).

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of extracts of 293T cells, using NADPH oxidase 4 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 90s.

Western blot analysis of extracts from normal and NADPH oxidase 4 Rabbit pAb knockout HeLa cells, using NADPH oxidase 4 Rabbit pAb antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 60s.

Immunohistochemistry of paraffin-embedded human kidney using [KO Validated] NADPH oxidase 4 Rabbit pAb at dilution of 1:100 .Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Immunohistochemistry of paraffin-embedded mouse colon using [KO Validated] NADPH oxidase 4 Rabbit pAb at dilution of 1:100 .Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Astragaloside IV prevents kidney injury caused by iatrogenic hyperinsulinemia in a streptozotocin‑induced diabetic rat model

JournalInternational Journal Of Molecular Medicine

ApplicationWB

IF3.098

SpeciesRat

PMID29207011
Nox4 has a crucial role in uric acid‑induced oxidative stress and apoptosis in renal tubular cells

JournalMolecular Medicine Reports

ApplicationWB

IF2.1

SpeciesHuman

PMID
Lipophilic Extract and Tanshinone IIA Derived from Salvia miltiorrhiza Attenuate Uric Acid Nephropathy through Suppressing Oxidative Stress-Activated MAPK Pathways

JournalAmerican Journal Of Chinese Medicine

ApplicationWB

IF3.682

SpeciesMouse,Human

PMID32933312