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[KO Validated] Peroxiredoxin 4 (PRDX4) polyclonal antibody

货号: BS6787

价格：￥ 1140

货期：现货（1-2个工作日）

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说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :BS6787

Product Name :[KO Validated] Peroxiredoxin 4 (PRDX4) polyclonal antibody

Swiss-Prot :Q13162

Host :Rabbit

Reactivity :Human, Mouse, Rat

Applications :WB, IF/ICC

Application_all :WB,1:500 - 1:2000|IF/ICC,1:50 - 1:100

Background :The protein encoded by this gene is an antioxidant enzyme and belongs to the peroxiredoxin family. The protein is localized to the cytoplasm. Peroxidases of the peroxiredoxin family reduce hydrogen peroxide and alkyl hydroperoxides to water and alcohol with the use of reducing equivalents derived from thiol-containing donor molecules. This protein has been found to play a regulatory role in the activation of the transcription factor NF-kappaB.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Polyclonal Antibodies

BiowMW :28kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :PRDX4;AOE37-2;AOE372;HEL-S-97n;PRX-4

Immunogen :Recombinant fusion protein of human Peroxiredoxin 4 (Peroxiredoxin 4 (PRDX4))(NP_006397.1).

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of extracts of various cell lines, using Peroxiredoxin 4 ) antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

Western blot analysis of extracts from normal and Peroxiredoxin 4 ) knockout 293T cells, using Peroxiredoxin 4 ) antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 5s.

Sulfiredoxin‐1 protects PC12 cells against oxidative stress induced by hydrogen peroxide

JournalJournal Of Neuroscience Research

ApplicationWB

IF4.699

SpeciesRat

PMID23553940
Protective effect of curcumin against irinotecan‑induced intestinal mucosal injury via attenuation of NF‑κB activation, oxidative stress and endoplasmic reticulum stress

JournalInternational Journal Of Oncology

ApplicationIHC

IF3.571

SpeciesMouse

PMID30968152