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HDAC6 polyclonal antibody

货号: BS6784

价格：￥ 1140

货期：现货（1-2个工作日）

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No. :BS6784

Product Name :HDAC6 polyclonal antibody

Swiss-Prot :Q9UBN7

Host :Rabbit

Reactivity :Human, Mouse, Rat

Applications :WB, IHC, IF/ICC, IP

Application_all :WB,1:500 - 1:2000|IHC,1:50 - 1:200|IF/ICC,1:50 - 1:200|IP,1:20 - 1:50

Background :Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene belongs to class II of the histone deacetylase/acuc/apha family. It contains an internal duplication of two catalytic domains which appear to function independently of each other. This protein possesses histone deacetylase activity and represses transcription.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Polyclonal Antibodies

BiowMW :160KDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :HDAC6;CPBHM;HD6;JM21;PPP1R90

Immunogen :Recombinant fusion protein of human HDAC6(NP_006035.2).

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of extracts of Rat liver, using HDAC6 antibody at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 1s.

Immunohistochemistry of paraffin-embedded human liver using HDAC6 Rabbit pAb at dilution of 1:400 .Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Immunofluorescence analysis of HeLa cells using HDAC6 antibody at dilution of 1:100 . Blue: DAPI for nuclear staining.

Immunoprecipitation analysis of 100ug extracts of HepG2 cells using 3ug HDAC6 antibody . Western blot was performed from the immunoprecipitate using HDAC6 antibody at a dilution of 1:1000.

Expression patterns of histone deacetylases in experimental stroke and potential targets for neuroprotection

JournalClinical And Experimental Pharmacology And Physiology

ApplicationWB,IF

IF2.336

SpeciesMouse

PMID22651689
HDAC6 alleviates prion peptide-mediated neuronal death via modulating PI3K-Akt-mTOR pathway

JournalNeurobiology Of Aging

ApplicationWB,IF

IF4.398

SpeciesMouse

PMID26507311