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Tyrosinase polyclonal antibody

货号: BS6754

价格：￥ 1140

货期：现货（1-2个工作日）

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No. :BS6754

Product Name :Tyrosinase polyclonal antibody

Swiss-Prot :P14679

Host :Rabbit

Reactivity :Human, Mouse, Rat

Applications :WB, IHC

Application_all :WB,1:500 - 1:2000|IHC,1:50 - 1:200

Background :The enzyme encoded by this gene catalyzes the first 2 steps, and at least 1 subsequent step, in the conversion of tyrosine to melanin. The enzyme has both tyrosine hydroxylase and dopa oxidase catalytic activities, and requires copper for function. Mutations in this gene result in oculocutaneous albinism, and nonpathologic polymorphisms result in skin pigmentation variation. The human genome contains a pseudogene similar to the 3' half of this gene.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Polyclonal Antibodies

BiowMW :80KDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :TYR;ATN;CMM8;OCA1;OCA1A;OCAIA;SHEP3

Immunogen :A synthetic peptide of human Tyrosinase(NP_000363.1).

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of extracts of Rat brain, using Tyrosinase antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 90s.

Immunohistochemistry of paraffin-embedded rat heart using Tyrosinase antibody at dilution of 1:100 .Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Flavonoids, apigenin and icariin exert potent melanogenic activities in murine B16 melanoma cells

JournalPhytomedicine

ApplicationWB

IF4.268

SpeciesMouse

PMID20638260
SCF/c-kit signaling is required in 12-O-tetradecanoylphorbol-13-acetate-induced migration and differentiation of hair follicle melanocytes for epidermal pigmentation

JournalCell And Tissue Research

ApplicationIF,WB

IF4.044

SpeciesMouse

PMID
Inhibition of melanogenesis by jineol from Scolopendra subspinipes mutilans via MAP-Kinase mediated MITF downregulation and the proteasomal degradation of tyrosinase

JournalScientific Reports

ApplicationWB

IF3.998

SpeciesMouse

PMID28393917