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LKB1 polyclonal antibody

货号: BS6740

价格：￥ 1140

货期：现货（1-2个工作日）

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No. :BS6740

Product Name :LKB1 polyclonal antibody

Swiss-Prot :Q15831

Host :Rabbit

Reactivity :Human, Mouse, Rat

Applications :WB, IHC, IF/ICC

Application_all :WB,1:500 - 1:2000|IHC,1:50 - 1:200|IF/ICC,1:50 - 1:200

Background :This gene, which encodes a member of the serine/threonine kinase family, regulates cell polarity and functions as a tumor suppressor. Mutations in this gene have been associated with Peutz-Jeghers syndrome, an autosomal dominant disorder characterized by the growth of polyps in the gastrointestinal tract, pigmented macules on the skin and mouth, and other neoplasms. Alternate transcriptional splice variants of this gene have been observed but have not been thoroughly characterized.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Polyclonal Antibodies

BiowMW :49-60KDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :LKB1;PJS;hLKB1;STK11

Immunogen :Recombinant fusion protein of human LKB1(NP_000446.1).

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of various lysates, using LKB1 antibody at 1:690 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 30s.

Western blot analysis of Mouse heart, using LKB1 antibody at 1:690 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 30s.

Immunohistochemistry of paraffin-embedded human lung cancer using LKB1 Rabbit pAb at dilution of 1:50 .Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Immunofluorescence analysis of NIH/3T3 cells using LKB1 Rabbit pAb at dilution of 1:50 . Blue: DAPI for nuclear staining.

Fluoxetine suppresses AMP-activated protein kinase signaling pathway to promote hepatic lipid accumulation in primary mouse hepatocytes

JournalInternational Journal Of Biochemistry & Cell Biology

ApplicationWB,IF

IF3.144

SpeciesMouse

PMID
Decreased Expression of LKB1 Correlates with Poor Prognosis in Hepatocellular Carcinoma Patients Undergoing Hepatectomy

JournalAsian Pacific Journal of Cancer Prevention

ApplicationIHC

IF0

SpeciesHuman

PMID23679304