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SPAM1 polyclonal antibody

货号: BS6737
价格 1140
货期 现货(1-2个工作日)
25ul 50ul 100ul 200ul
No. :BS6737
Product Name :SPAM1 polyclonal antibody
Swiss-Prot :P38567
Host :Rabbit
Reactivity :Human, Mouse, Rat
Applications :WB, IHC, IF/ICC
Application_all :WB,1:500 - 1:2000|IHC,1:50 - 1:200|IF/ICC,1:50 - 1:200
Background :Hyaluronidase degrades hyaluronic acid, a major structural proteoglycan found in extracellular matrices and basement membranes. Six members of the hyaluronidase family are clustered into two tightly linked groups on chromosome 3p21.3 and 7q31.3. This gene was previously referred to as HYAL1 and HYA1 and has since been assigned the official symbol SPAM1; another family member on chromosome 3p21.3 has been assigned HYAL1. This gene encodes a GPI-anchored enzyme located on the human sperm surface and inner acrosomal membrane. This multifunctional protein is a hyaluronidase that enables sperm to penetrate through the hyaluronic acid-rich cumulus cell layer surrounding the oocyte, a receptor that plays a role in hyaluronic acid induced cell signaling, and a receptor that is involved in sperm-zona pellucida adhesion. Abnormal expression of this gene in tumors has implicated this protein in degradation of basement membranes leading to tumor invasion and metastasis. Multiple transcript variants encoding different isoforms have been found for this gene.
Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity :Polyclonal Antibodies
BiowMW :64KDa
Note :For research use only, not for use in diagnostic procedure.
Alternative Name :SPAM1;HEL-S-96n;HYA1;HYAL1;HYAL3;HYAL5;PH-20;PH20;SPAG15
Immunogen :Recombinant fusion protein of human SPAM1(NP_003108.2).
Conjugate :Unconjugated
Modification :Unmodification
Western blot analysis of extracts of various cell lines, using SPAM1 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 10s.
Immunohistochemistry of paraffin-embedded rat testis using SPAM1 Rabbit pAb at dilution of 1:100 .Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunofluorescence analysis of rat testis cells using SPAM1 Rabbit pAb at dilution of 1:100 . Blue: DAPI for nuclear staining.
Immunofluorescence analysis of human testis cells using SPAM1 Rabbit pAb at dilution of 1:150 . Blue: DAPI for nuclear staining.

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400-660-9091

电话:025-68037686

地址:江苏生命科技创新园F6幢1层

订购:nanjing03@biogot.com

服务:biorase01@biogot.com

合作:lvyun@biogot.com

支持:may@biogot.com