Western blot analysis of Collagen 10 alpha 1 expression in MCF7 (A) whole cell lysates.
Immunohistochemical analysis of Collagen 10 alpha 1 staining in human liver cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.