产品类别

联系我们

电话:025-68037686

地址:江苏生命科技创新园F6幢1层

订购:nanjing03@biogot.com

服务:biorase01@biogot.com

合作:lvyun@biogot.com

支持:may@biogot.com

SOD2 polyclonal antibody

货号: BS6734

价格：￥ 1140

货期：现货（1-2个工作日）

25ul 50ul 100ul 200ul

抗体定制服务咨询

说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :BS6734

Product Name :SOD2 polyclonal antibody

Swiss-Prot :P04179

Host :Rabbit

Reactivity :Human, Mouse, Rat

Applications :WB, IHC, IF/ICC

Application_all :WB,1:500 - 1:2000|IHC,1:50 - 1:200|IF/ICC,1:50 - 1:200

Background :This gene is a member of the iron/manganese superoxide dismutase family. It encodes a mitochondrial protein that forms a homotetramer and binds one manganese ion per subunit. This protein binds to the superoxide byproducts of oxidative phosphorylation and converts them to hydrogen peroxide and diatomic oxygen. Mutations in this gene have been associated with idiopathic cardiomyopathy (IDC), premature aging, sporadic motor neuron disease, and cancer. Alternative splicing of this gene results in multiple transcript variants. A related pseudogene has been identified on chromosome 1.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Polyclonal Antibodies

BiowMW :25KDa/22KDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :IPO-B;IPOB;MNSOD;MVCD6;Mn-SOD;SOD2

Immunogen :Recombinant fusion protein of human SOD2(NP_000627.2).

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of extracts of various cell lines, using SOD2 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 10s.

Immunohistochemistry of paraffin-embedded rat ovary using [KO Validated] SOD2 Rabbit pAb at dilution of 1:100 .Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Immunofluorescence analysis of C6 cells using [KO Validated] SOD2 Rabbit pAb at dilution of 1:200 . Blue: DAPI for nuclear staining.

Oleanolic acid improves hepatic insulin resistance via antioxidant, hypolipidemic and anti-inflammatory effects

JournalMolecular And Cellular Endocrinology

ApplicationWB

IF3.693

SpeciesMouse

PMID23791844
Depletion of NFBD1/MDC1 Induces Apoptosis in Nasopharyngeal Carcinoma Cells Through the p53–ROS–Mitochondrial Pathway

JournalOncology Research

ApplicationWB

IF5.574

SpeciesHuman

PMID28081741
Peroxisome Proliferator-Activated Receptor-γ Coactivator-1α Inhibits Vascular Calcification Through Sirtuin 3-Mediated Reduction of Mitochondrial Oxidative Stress

JournalAntioxidants & Redox Signaling

ApplicationWB

IF5.828

SpeciesRat

PMID30829051
Inhibition of mitochondrial complex I by rotenone protects against acetaminophen-induced liver injury

JournalAmerican Journal of Translational Research

ApplicationWB

IF3.375

SpeciesMouse

PMID30787978
JNK and Jag1/Notch2 co-regulate CXCL16 to facilitate cypermethrin-induced kidney damage

JournalECOTOXICOLOGY AND ENVIRONMENTAL SAFETY

ApplicationWB

IF6.291

SpeciesRat

PMID35512476