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[KO Validated] RBBP4 polyclonal antibody

货号: BS6721

价格：￥ 1140

货期：现货（1-2个工作日）

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说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :BS6721

Product Name :[KO Validated] RBBP4 polyclonal antibody

Swiss-Prot :Q09028

Host :Rabbit

Reactivity :Human

Applications :WB, IF/ICC, ChIP

Application_all :WB,1:500 - 1:2000|IF/ICC,1:50 - 1:200|ChIP,1:20 - 1:50

Background :This gene encodes a ubiquitously expressed nuclear protein which belongs to a highly conserved subfamily of WD-repeat proteins. It is present in protein complexes involved in histone acetylation and chromatin assembly. It is part of the Mi-2 complex which has been implicated in chromatin remodeling and transcriptional repression associated with histone deacetylation. This encoded protein is also part of co-repressor complexes, which is an integral component of transcriptional silencing. It is found among several cellular proteins that bind directly to retinoblastoma protein to regulate cell proliferation. This protein also seems to be involved in transcriptional repression of E2F-responsive genes. Three transcript variants encoding different isoforms have been found for this gene.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Polyclonal Antibodies

BiowMW :48KDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :RBBP4;NURF55;RBAP48;lin-53

Immunogen :Recombinant fusion protein of human RBBP4(NP_005601.1).

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of extracts from normal and RBBP4 knockout 293T cells, using RBBP4 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 1s.

Chromatin immunoprecipitation analysis of extracts of 293T cells, using RBBP4 antibody and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.