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RAD9A polyclonal antibody

货号: BS6718
价格 1140
货期 现货(1-2个工作日)
25ul 50ul 100ul 200ul
No. :BS6718
Product Name :RAD9A polyclonal antibody
Swiss-Prot :Q99638
Host :Rabbit
Reactivity :Human, Rat
Applications :WB, IHC
Application_all :WB,1:500 - 1:2000|IHC,1:50 - 1:200
Background :This gene product is highly similar to Schizosaccharomyces pombe rad9, a cell cycle checkpoint protein required for cell cycle arrest and DNA damage repair. This protein possesses 3' to 5' exonuclease activity, which may contribute to its role in sensing and repairing DNA damage. It forms a checkpoint protein complex with RAD1 and HUS1. This complex is recruited by checkpoint protein RAD17 to the sites of DNA damage, which is thought to be important for triggering the checkpoint-signaling cascade. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity :Polyclonal Antibodies
BiowMW :60kDa
Note :For research use only, not for use in diagnostic procedure.
Alternative Name :RAD9A;RAD9
Immunogen :Recombinant fusion protein of human RAD9A(NP_004575.1).
Conjugate :Unconjugated
Modification :Unmodification
Western blot analysis of extracts of various cell lines, using RAD9A antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Immunohistochemistry of paraffin-embedded rat liver using RAD9A antibody at dilution of 1:200 .Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunohistochemistry of paraffin-embedded human liver damage using RAD9A antibody at dilution of 1:200 .Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
  • Knockdown of Rad9A enhanced DNA damage induced by trichostatin A in esophageal cancer cells
    JournalTumor Biology
    ApplicationWB
    IF0
    SpeciesHuman

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400-660-9091

电话:025-68037686

地址:江苏生命科技创新园F6幢1层

订购:nanjing03@biogot.com

服务:biorase01@biogot.com

合作:lvyun@biogot.com

支持:may@biogot.com