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RagA/B polyclonal antibody

货号: BS67084

价格：￥ 1140

货期：现货（1-2个工作日）

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No. :BS67084

Product Name :RagA/B polyclonal antibody

Swiss-Prot :Q7L523; Q5VZM2

Host :Rabbit

Reactivity :Human, Mouse, Rat

Applications :WB, IHC

Application_all :WB (1/500 - 1/1000), IH (1/100 - 1/200)

Background :Guanine nucleotide-binding protein that plays a crucial role in the cellular response to amino acid availability through regulation of the mTORC1 signaling cascade (PubMed:20381137, PubMed:24095279, PubMed:25936802).

Product :Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Recognizes endogenous levels of RagA/B protein.

BiowMW :~ 35 kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :RRAGA; Ras-related GTP-binding protein A; Rag A; RagA; Adenovirus E3 14.7 kDa-interacting protein 1; FIP-1; RRAGB; Ras-related GTP-binding protein B; Rag B; RagB

Immunogen :KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human RagA/B. The exact sequence is proprietary.

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of RagA/B expression in mouse lung (A), rat testis (B) whole cell lysates.

Immunohistochemical analysis of RagA/B staining in human prostate cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.