中国 EN 400-660-9091

联系我们

电话:025-68037686

地址:江苏生命科技创新园F6幢1层

订购:nanjing03@biogot.com

服务:biorase01@biogot.com

合作:lvyun@biogot.com

支持:may@biogot.com

PKC epsilon polyclonal antibody

货号: BS6704
价格 1140
货期 现货(1-2个工作日)
25ul 50ul 100ul 200ul
No. :BS6704
Product Name :PKC epsilon polyclonal antibody
Swiss-Prot :Q02156
Host :Rabbit
Reactivity :Human, Mouse, Rat
Applications :WB, IHC
Application_all :WB,1:500 - 1:2000|IHC,1:50 - 1:200
Background :Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. The protein encoded by this gene is one of the PKC family members. This kinase has been shown to be involved in many different cellular functions, such as neuron channel activation, apoptosis, cardioprotection from ischemia, heat shock response, as well as insulin exocytosis. Knockout studies in mice suggest that this kinase is important for lipopolysaccharide (LPS)-mediated signaling in activated macrophages and may also play a role in controlling anxiety-like behavior.
Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity :Polyclonal Antibodies
BiowMW :100kDa
Note :For research use only, not for use in diagnostic procedure.
Alternative Name :PRKCE;PKCE;nPKC-epsilon
Immunogen :Recombinant fusion protein of human PKC epsilon(NP_005391.1).
Conjugate :Unconjugated
Modification :Unmodification
Western blot analysis of extracts of various cell lines, using PKC epsilon antibody at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Immunohistochemistry of paraffin-embedded rat spleen using PKC epsilon antibody at dilution of 1:200 .Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunohistochemistry of paraffin-embedded human esophageal cancer using PKC epsilon antibody at dilution of 1:200 .Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunohistochemistry of paraffin-embedded human stomach using PKC epsilon antibody at dilution of 1:100 .Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
  • Metformin Inhibits Angiotensin II-Induced Differentiation of Cardiac Fibroblasts into Myofibroblasts
    JournalPLoS One
    ApplicationWB
    IF2.74
    SpeciesRat
    PMID
  • Gondoic acid alleviates LPS‑induced Kupffer cells inflammation by inhibiting ROS production and PKCθ/ERK/STAT3 signaling pathway
    JournalINTERNATIONAL IMMUNOPHARMACOLOGY
    ApplicationWB
    IF5.714
    SpeciesMouse
  • Ginseng oligosaccharides (GSO) inhibit C48/80-stimulated pseudoallergic mechanisms through the PLC/Ca2+/PKC/MAPK/c-Fos signaling pathway
    JournalJournal of Functional Foods
    ApplicationWB
    IF5.223
    SpeciesRat
    PMID

关注我们

400-660-9091

电话:025-68037686

地址:江苏生命科技创新园F6幢1层

订购:nanjing03@biogot.com

服务:biorase01@biogot.com

合作:lvyun@biogot.com

支持:may@biogot.com