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DNA polymerase eta polyclonal antibody

货号: BS6695

价格：￥ 1140

货期：现货（1-2个工作日）

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详细信息检测数据实验指南文献

No. :BS6695

Product Name :DNA polymerase eta polyclonal antibody

Swiss-Prot :Q9Y253

Host :Rabbit

Reactivity :Human, Mouse, Rat

Applications :WB, IF/ICC

Application_all :WB,1:500 - 1:2000|IF/ICC,1:50 - 1:200

Background :This gene encodes a member of the Y family of specialized DNA polymerases. It copies undamaged DNA with a lower fidelity than other DNA-directed polymerases. However, it accurately replicates UV-damaged DNA; when thymine dimers are present, this polymerase inserts the complementary nucleotides in the newly synthesized DNA, thereby bypassing the lesion and suppressing the mutagenic effect of UV-induced DNA damage. This polymerase is thought to be involved in hypermutation during immunoglobulin class switch recombination. Mutations in this gene result in XPV, a variant type of xeroderma pigmentosum. Several transcript variants encoding different isoforms have been found for this gene.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Polyclonal Antibodies

BiowMW :80KDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :POLH;RAD30;RAD30A;XP-V;XPV

Immunogen :Recombinant fusion protein of human DNA polymerase eta(NP_006493.1).

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of extracts of various cell lines, using DNA polymerase eta antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 180s.

Immunofluorescence analysis of U-2 OS cells using DNA polymerase eta Rabbit pAb at dilution of 1:100 . Blue: DAPI for nuclear staining.

NEDDylation antagonizes ubiquitination of proliferating cell nuclear antigen and regulates the recruitment of polymerase η in response to oxidative DNA damage

JournalProtein & Cell

ApplicationIF,CO-IP

IF14.87

SpeciesHuman

PMID28831681
The herpes simplex virus 1 UL36USP deubiquitinase suppresses DNA repair in host cells via deubiquitination of proliferating cell nuclear antigen

JournalJournal Of Biological Chemistry

ApplicationIF

IF5.157

SpeciesHuman

PMID28348081
NEDDylation regulates RAD18 ubiquitination and localization in response to oxidative DNA damage

JournalBiochemical And Biophysical Research Communications

ApplicationWB

IF2.985

SpeciesHuman

PMID