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[KO Validated] MMP13 polyclonal antibody

货号: BS6668

价格：￥ 1140

货期：现货（1-2个工作日）

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No. :BS6668

Product Name :[KO Validated] MMP13 polyclonal antibody

Swiss-Prot :P45452

Host :Rabbit

Reactivity :Human, Mouse, Rat

Applications :WB, IF/ICC

Application_all :WB,1:500 - 1:2000|IF/ICC,1:50 - 1:200

Background :This gene encodes a member of the peptidase M10 family of matrix metalloproteinases (MMPs). Proteins in this family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. The encoded preproprotein is proteolytically processed to generate the mature protease. This protease cleaves type II collagen more efficiently than types I and III. It may be involved in articular cartilage turnover and cartilage pathophysiology associated with osteoarthritis. Mutations in this gene are associated with metaphyseal anadysplasia. This gene is part of a cluster of MMP genes on chromosome 11.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Polyclonal Antibodies

BiowMW :65-70KDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :MMP13;CLG3;MANDP1;MDST;MMP-13

Immunogen :Recombinant fusion protein of human MMP13(NP_002418.1).

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of extracts of various cell lines, using MMP13 Rabbit pAb at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 3s.

Western blot analysis of various lysates, using MMP13 antibody at 1:2900 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 90s.

Western blot analysis of extracts from wild type and MMP13 knockdown 293T cells, using MMP13 antibody at 1:2900 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 90s.

Immunofluorescence analysis of C6 cells using MMP13 Rabbit pAb at dilution of 1:100 . Blue: DAPI for nuclear staining.

Trichostatin A increases the TIMP-1/MMP ratio to protect against osteoarthritis in an animal model of the disease

JournalMolecular Medicine Reports

ApplicationWB

IF1.851

SpeciesRat

PMID27431944
Protective effect of glucosamine cyclohexyl ester on osteoarthritis in rat via targeting expressions of matrix metalloproteinase and tissue inhibitor of metalloproteinases-1

JournalTropical Journal Of Pharmaceutical Research

ApplicationWB

IF0.504

SpeciesRat

PMID
17β-Estradiol inhibits intervertebral disc degeneration by down-regulating MMP-3 and MMP-13 and up-regulating type II collagen in a rat model

JournalArtificial Cells Nanomedicine and Biotechnology

ApplicationWB

IF4.462

SpeciesRat

PMID30056756
Phenyllactic acid promotes cell migration and invasion in cervical cancer via IKK/NF-κB-mediated MMP-9 activation

JournalCancer Cell International

ApplicationWB

IF3.439

SpeciesHuman

PMID31572058
Ergosterol limits osteoarthritis development and progression through activation of Nrf2 signaling

JournalExperimental and Therapeutic Medicine

ApplicationWB

IF1.785

SpeciesMouse

PMID33488803