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MITF polyclonal antibody

货号: BS6666

价格：￥ 1140

货期：现货（1-2个工作日）

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详细信息检测数据实验指南文献

No. :BS6666

Product Name :MITF polyclonal antibody

Swiss-Prot :O75030

Host :Rabbit

Reactivity :Human, Mouse

Applications :WB

Application_all :WB,1:500 - 1:2000

Background :This gene encodes a transcription factor that contains both basic helix-loop-helix and leucine zipper structural features. It regulates the differentiation and development of melanocytes retinal pigment epithelium and is also responsible for pigment cell-specific transcription of the melanogenesis enzyme genes. Heterozygous mutations in the this gene cause auditory-pigmentary syndromes, such as Waardenburg syndrome type 2 and Tietz syndrome. Alternatively spliced transcript variants encoding different isoforms have been identified.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Polyclonal Antibodies

BiowMW :50-70KDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :MITF;CMM8;COMMAD;MI;WS2;WS2A;bHLHe32

Immunogen :Recombinant fusion protein of human MITF(NP_937801.1).

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of extracts of various cell lines, using MITF antibody at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 180s.

Involvement of p38 MAPK signaling pathway in the anti-melanogenic effect of San-bai-tang, a Chinese herbal formula, in B16 cells

JournalJournal Of Ethnopharmacology

ApplicationWB

IF3.69

SpeciesMouse

PMID
Alcohol extract from Vernonia anthelmintica (L.) willd seed enhances melanin synthesis through activation of the p38 MAPK signaling pathway in B16F10 cells and primary melanocytes

JournalJournal Of Ethnopharmacology

ApplicationWB

IF3.69

SpeciesMouse,Human

PMID
Cilostazol promotes production of melanin by activating the microphthalmia-associated transcription factor (MITF)

JournalBiochemical And Biophysical Research Communications

ApplicationWB

IF2.985

SpeciesMouse

PMID24333333
Attenuation of melanogenesis by Nymphaea nouchali (Burm. f) flower extract through the regulation of cAMP/CREB/MAPKs/MITF and proteasomal degradation of tyrosinase

JournalScientific Reports

ApplicationWB

IF3.998

SpeciesMouse

PMID30224716
Fuzhuan Brick Tea Boosts Melanogenesis and Prevents Hair Graying through Reduction of Oxidative Stress via NRF2-HO-1 Signaling

JournalAntioxidants

ApplicationWB

IF6.312

SpeciesMouse

PMID35326249