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LIG1 polyclonal antibody

货号: BS6652

价格：￥ 1140

货期：现货（1-2个工作日）

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No. :BS6652

Product Name :LIG1 polyclonal antibody

Swiss-Prot :P18858

Host :Rabbit

Reactivity :Human, Mouse

Applications :WB, IHC, IF/ICC

Application_all :WB,1:500 - 1:2000|IHC,1:50 - 1:200|IF/ICC,1:50 - 1:200

Background :This gene encodes a member of the ATP-dependent DNA ligase protein family. The encoded protein functions in DNA replication, recombination, and the base excision repair process. Mutations in this gene that lead to DNA ligase I deficiency result in immunodeficiency and increased sensitivity to DNA-damaging agents. Disruption of this gene may also be associated with a variety of cancers. Alternative splicing results in multiple transcript variants.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Polyclonal Antibodies

BiowMW :140KDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :LIG1

Immunogen :Recombinant fusion protein of human LIG1(NP_000225.1).

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of extracts of various cell lines, using LIG1 Rabbit pAb at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 1s.

Immunofluorescence analysis of MCF-7 cells using LIG1 antibody . Blue: DAPI for nuclear staining.

Immunofluorescence analysis of A549 cells using LIG1 antibody .