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Cytokeratin 17 (KRT17) polyclonal antibody

货号: BS6649

价格：￥ 1140

货期：现货（1-2个工作日）

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说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :BS6649

Product Name :Cytokeratin 17 (KRT17) polyclonal antibody

Swiss-Prot :Q04695

Host :Rabbit

Reactivity :Human, Mouse

Applications :WB, IF/ICC

Application_all :WB,1:500 - 1:2000|IF/ICC,1:50 - 1:200

Background :This gene encodes the type I intermediate filament chain keratin 17, expressed in nail bed, hair follicle, sebaceous glands, and other epidermal appendages. Mutations in this gene lead to Jackson-Lawler type pachyonychia congenita and steatocystoma multiplex.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Polyclonal Antibodies

BiowMW :48KDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :KRT17;39.1;CK-17;K17;PC;PC2;PCHC1

Immunogen :A synthetic peptide of human Cytokeratin 17 (KRT17)(NP_000413.1).

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of extracts of various cell lines, using Cytokeratin 17 antibody at 1:1298 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 1s.

Western blot analysis of extracts of Mouse stomach, using Cytokeratin 17 antibody at 1:1298 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 60s.

Confocal immunofluorescence analysis of HeLa cells using Cytokeratin 17 antibody at dilution of 1:200. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of HeLa cells using Cytokeratin 17 Rabbit pAb at dilution of 1:200 . Blue: DAPI for nuclear staining.