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EIF6 polyclonal antibody

货号: BS6641
价格 1140
货期 现货(1-2个工作日)
25ul 50ul 100ul 200ul
No. :BS6641
Product Name :EIF6 polyclonal antibody
Swiss-Prot :P56537
Host :Rabbit
Reactivity :Human
Applications :WB, IF/ICC
Application_all :WB,1:500 - 1:2000|IF/ICC,1:50 - 1:200
Background :Hemidesmosomes are structures which link the basal lamina to the intermediate filament cytoskeleton. An important functional component of hemidesmosomes is the integrin beta-4 subunit (ITGB4), a protein containing two fibronectin type III domains. The protein encoded by this gene binds to the fibronectin type III domains of ITGB4 and may help link ITGB4 to the intermediate filament cytoskeleton. The encoded protein, which is insoluble and found both in the nucleus and in the cytoplasm, can function as a translation initiation factor and prevent the association of the 40S and 60S ribosomal subunits. Multiple non-protein coding transcript variants and variants encoding two different isoforms have been found for this gene.
Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity :Polyclonal Antibodies
BiowMW :27kDa
Note :For research use only, not for use in diagnostic procedure.
Alternative Name :EIF6;CAB;EIF3A;ITGB4BP;b(2)gcn;eIF-6;p27(BBP);p27BBP
Immunogen :Recombinant fusion protein of human EIF6(NP_852133.1).
Conjugate :Unconjugated
Modification :Unmodification
Western blot analysis of extracts of various cell lines, using EIF6 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Immunofluorescence analysis of MCF-7 cells using EIF6 antibody . Blue: DAPI for nuclear staining.
Confocal immunofluorescence analysis of Hela cells using EIF6 antibody at dilution of 1:50. Blue: DAPI for nuclear staining.
Confocal immunofluorescence analysis of U-2OS cells using EIF6 antibody at dilution of 1:50. Blue: DAPI for nuclear staining.

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400-660-9091

电话:025-68037686

地址:江苏生命科技创新园F6幢1层

订购:nanjing03@biogot.com

服务:biorase01@biogot.com

合作:lvyun@biogot.com

支持:may@biogot.com