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CD11b/ITGAM polyclonal antibody

货号: BS6640

价格：￥ 1140

货期：现货（1-2个工作日）

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说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :BS6640

Product Name :CD11b/ITGAM polyclonal antibody

Swiss-Prot :P11215

Host :Rabbit

Reactivity :Human, Mouse, Rat

Applications :WB, IHC, IF/ICC

Application_all :WB,1:500 - 1:2000|IHC,1:50 - 1:200|IF/ICC,1:50 - 1:200

Background :This gene encodes the integrin alpha M chain. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This I-domain containing alpha integrin combines with the beta 2 chain (ITGB2) to form a leukocyte-specific integrin referred to as macrophage receptor 1 ('Mac-1'), or inactivated-C3b (iC3b) receptor 3 ('CR3'). The alpha M beta 2 integrin is important in the adherence of neutrophils and monocytes to stimulated endothelium, and also in the phagocytosis of complement coated particles. Multiple transcript variants encoding different isoforms have been found for this gene.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Polyclonal Antibodies

BiowMW :170KDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :CD11B;CR3A;MAC-1;MAC1A;MO1A;SLEB6;ITGAM;CD11b/ITAM

Immunogen :Recombinant fusion protein of human CD11b/ITGAM(NP_000623.2).

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of extracts of various cell lines, using CD11b/ITGAM antibody at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit .
Exposure time: 90s.

Immunohistochemistry of paraffin-embedded human tonsil using CD11b/ITGAM antibody at dilution of 1:100 .Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Immunohistochemistry of paraffin-embedded mouse spleen using CD11b/ITGAM antibody at dilution of 1:100 .Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Immunofluorescence analysis of TF-1 cells using CD11b/ITGAM Rabbit pAb at dilution of 1:100 . Blue: DAPI for nuclear staining.

The Neuroprotective Effect of Short Chain Fatty Acids Against Sepsis-Associated Encephalopathy in Mice

JournalFrontiers in Immunology

ApplicationIF, WB

IF7.561

SpeciesMouse

PMID33584734
Effect of Probiotic Fungi against Cognitive Impairment in Mice via Regulation of the Fungal Microbiota–Gut–Brain Axis

JournalJOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY

ApplicationIF,WB

IF5.895

SpeciesMouse

PMID35833673
Gut commensal Agathobacter rectalis alleviates microglia-mediated neuroinflammation against pathogenesis of Alzheimer’s disease

Journal10.1016/j.isci.2024.111116

ApplicationWB

IF4.6

SpeciesMouse

PMID39498309
Epidural injection of varying doses of capsaicin alleviates inflammatory pain in rats via the TLR4/AKT/NF-κB pathway

Journal10.1007/s10787-024-01617-6

ApplicationIF

IF4.6

SpeciesRat

PMID39690361