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IGF2 polyclonal antibody

货号: BS6632
价格 1140
货期 现货(1-2个工作日)
25ul 50ul 100ul 200ul
No. :BS6632
Product Name :IGF2 polyclonal antibody
Swiss-Prot :P01344
Host :Rabbit
Reactivity :Human, Mouse, Rat
Applications :WB, IHC, IF/ICC
Application_all :WB,1:500 - 1:2000|IHC,1:50 - 1:200|IF/ICC,1:50 - 1:100
Background :This gene encodes a member of the insulin family of polypeptide growth factors, which are involved in development and growth. It is an imprinted gene, expressed only from the paternal allele, and epigenetic changes at this locus are associated with Wilms tumour, Beckwith-Wiedemann syndrome, rhabdomyosarcoma, and Silver-Russell syndrome. A read-through INS-IGF2 gene exists, whose 5' region overlaps the INS gene and the 3' region overlaps this gene. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity :Polyclonal Antibodies
BiowMW :20KDa
Note :For research use only, not for use in diagnostic procedure.
Alternative Name :IGF2;C11orf43;GRDF;IGF-II;PP9974
Immunogen :Recombinant fusion protein of human IGF2(P01344).
Conjugate :Unconjugated
Modification :Unmodification
Western blot analysis of extracts of HepG2 cells, using IGF2 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 180s.
Immunohistochemistry of paraffin-embedded human placenta using IGF2 Rabbit pAb at dilution of 1:100 .Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
  • Transgenerational Inheritance of Betaine‐Induced Epigenetic Alterations in Estrogen‐Responsive IGF‐2/IGFBP2 Genes in Rat Hippocampus
    JournalMolecular Nutrition & Food Research
    ApplicationWB
    IF5.309
    SpeciesRat
  • Mitotically Stable Modification of DNA Methylation in IGF2/H19 Imprinting Control Region Is Associated with Activated Hepatic IGF2 Expression in Offspring Rats from Betaine-Supplemented Dams
    JournalJournal Of Agricultural And Food Chemistry
    ApplicationWB
    IF3.571
    SpeciesRat
  • Grandmaternal betaine supplementation enhances hepatic IGF2 expression in F2 rat offspring through modification of promoter DNA methylation
    JournalJournal Of The Science Of Food And Agriculture
    ApplicationWB
    IF2.614
    SpeciesRat

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400-660-9091

电话:025-68037686

地址:江苏生命科技创新园F6幢1层

订购:nanjing03@biogot.com

服务:biorase01@biogot.com

合作:lvyun@biogot.com

支持:may@biogot.com