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HMGCR polyclonal antibody

货号: BS6625
价格 1140
货期 现货(1-2个工作日)
25ul 50ul 100ul 200ul
No. :BS6625
Product Name :HMGCR polyclonal antibody
Swiss-Prot :P04035
Host :Rabbit
Reactivity :Human, Mouse, Rat
Applications :WB, IHC, IF/ICC
Application_all :WB,1:1000 - 1:4000|IHC,1:50 - 1:100|IF/ICC,1:50 - 1:200
Background :HMG-CoA reductase is the rate-limiting enzyme for cholesterol synthesis and is regulated via a negative feedback mechanism mediated by sterols and non-sterol metabolites derived from mevalonate, the product of the reaction catalyzed by reductase. Normally in mammalian cells this enzyme is suppressed by cholesterol derived from the internalization and degradation of low density lipoprotein (LDL) via the LDL receptor. Competitive inhibitors of the reductase induce the expression of LDL receptors in the liver, which in turn increases the catabolism of plasma LDL and lowers the plasma concentration of cholesterol, an important determinant of atherosclerosis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity :Polyclonal Antibodies
BiowMW :97KDa
Note :For research use only, not for use in diagnostic procedure.
Alternative Name :HMGCR;LDLCQ3;HMGCR
Immunogen :A synthetic peptide of human HMGCR(NP_000850.1).
Conjugate :Unconjugated
Modification :Unmodification
Western blot analysis of extracts of Rat brain, using HMGCR Rabbit pAb at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 1s.
Western blot analysis of extracts of Mouse spleen, using HMGCR Rabbit pAb at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 10s.
Immunohistochemistry of paraffin-embedded human small intestine using HMGCR antibody at dilution of 1:200 .Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunohistochemistry of paraffin-embedded human uterine cancer using HMGCR antibody at dilution of 1:200 .Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
  • In Ovo Injection of Betaine Affects Hepatic Cholesterol Metabolism through Epigenetic Gene Regulation in Newly Hatched Chicks
    JournalPLoS One
    ApplicationWB
    IF2.776
    SpeciesChicken
  • Prenatal betaine exposure alleviates corticosterone-induced inhibition of CYP27A1 expression in the liver of juvenile chickens associated with its promoter DNA methylation
    JournalGeneral And Comparative Endocrinology
    ApplicationWB
    IF2.445
    SpeciesChicken
  • Prenatal betaine exposure modulates hypothalamic expression of cholesterol metabolic genes in cockerels through modifications of DNA methylation
    JournalPoultry Science
    ApplicationWB
    IF2.027
    SpeciesChicken
  • Activation of epithelial proliferation induced by Eimeria acervulina infection in the duodenum may be associated with cholesterol metabolism
    JournalOncotarget
    ApplicationWB
    IF0
    SpeciesChickens
  • Maternal betaine supplementation decreases hepatic cholesterol deposition in chicken offspring with epigenetic modulation of SREBP2 and CYP7A1 genes
    JournalPoultry Science
    ApplicationWB
    IF2.659
    SpeciesChicken

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400-660-9091

电话:025-68037686

地址:江苏生命科技创新园F6幢1层

订购:nanjing03@biogot.com

服务:biorase01@biogot.com

合作:lvyun@biogot.com

支持:may@biogot.com