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NFKB p65 (phospho-Ser536) polyclonal antibody

货号: BS66162

价格：￥ 1350

货期：现货（1-2个工作日）

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No. :BS66162

Product Name :NFKB p65 (phospho-Ser536) polyclonal antibody

Swiss-Prot :Q04206

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :WB IF/ICC IHC

Application_all :WB 1:500 - 1:1000 IHC 1:50 - 1:200 IF/ICC 1:50 - 1:200

Background :NF-kappa-B is a pleiotropic transcription factor present in almost all cell types and is the endpoint of a series of signal transduction events that are initiated by a vast array of stimuli related to many biological processes such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52 and the heterodimeric p65-p50 complex appears to be most abundant one. The dimers bind at kappa-B sites in the DNA of their target genes and the individual dimers have distinct preferences for different kappa-B sites that they can bind with distinguishable affinity and specificity. Different dimer combinations act as transcriptional activators or repressors, respectively. NF-kappa-B is controlled by various mechanisms of post-translational modification and subcellular compartmentalization as well as by interactions with other cofactors or corepressors. NF-kappa-B complexes are held in the cytoplasm in an inactive state complexed with members of the NF-kappa-B inhibitor (I-kappa-B) family. In a conventional activation pathway, I-kappa-B is phosphorylated by I-kappa-B kinases (IKKs) in response to different activators, subsequently degraded thus liberating the active NF-kappa-B complex which translocates to the nucleus. NF-kappa-B heterodimeric p65-p50 and p65-c-Rel complexes are transcriptional activators. The NF-kappa-B p65-p65 complex appears to be involved in invasin-mediated activation of IL-8 expression. The inhibitory effect of I-kappa-B upon NF-kappa-B the cytoplasm is exerted primarily through the interaction with p65. p65 shows a weak DNA-binding site which could contribute directly to DNA binding in the NF-kappa-B complex. Associates with chromatin at the NF-kappa-B promoter region via association with DDX1.

Product :Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :NFKB p65 (phospho-Ser536) polyclonal antibody detects endogenous levels of NFKB p65 protein only when phosphorylated at Ser536.

BiowMW :~ 70 kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :NFKB3; Transcription factor p65; Nuclear factor NF-kappa-B p65 subunit; Nuclear factor of kappa light polypeptide gene enhancer in B-cells 3

Immunogen :KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding S536 of human NF-kappaB p65 protein.

Conjugate :Unconjugated

Modification :Phosphorylated

Western blot analysis of NF-kappaB p65 (Phospho-S536) expression in Hela (A), HEK293T (B), NIH3T3 (C), mouse kidney (D), H9C2 (E) whole cell lysates.

Immunohistochemical analysis of NF-kappaB p65 (Phospho-S536) staining in human breast cancer formalin fixed paraffin embedded tissue section.

Immunofluorescent analysis of NF-kappaB p65 (Phospho-S536) staining in rat lung.

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