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SMOC1 polyclonal antibody

货号: BS66157

价格：￥ 1140

货期：现货（1-2个工作日）

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No. :BS66157

Product Name :SMOC1 polyclonal antibody

Swiss-Prot :Q9H4F8

Host :Rabbit

Reactivity :Human, Mouse

Applications :WB, IHC

Application_all :WB,1:500 - 1:2000|IHC,1:50 - 1:200

Background :This gene encodes a multi-domain secreted protein that may have a critical role in ocular and limb development. Mutations in this gene are associated with microphthalmia and limb anomalies. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Polyclonal Antibodies

BiowMW :48KDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :OAS

Immunogen :Recombinant fusion protein of human SMOC1(NP_071420.1).

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of extracts of Mouse liver, using SMOC1 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 10s.

Immunohistochemistry of paraffin-embedded human colon carcinoma using SMOC1 antibody at dilution of 1:250 .Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.