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[KO Validated] eIF4E polyclonal antibody

货号: BS6591

价格：￥ 1140

货期：现货（1-2个工作日）

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No. :BS6591

Product Name :[KO Validated] eIF4E polyclonal antibody

Swiss-Prot :P06730

Host :Rabbit

Reactivity :Human, Mouse, Rat

Applications :WB, IHC, IF/ICC, IP

Application_all :WB,1:500 - 1:2000|IHC,1:50 - 1:100|IF/ICC,1:50 - 1:200|IP,1:50 - 1:100

Background :The protein encoded by this gene is a component of the eukaryotic translation initiation factor 4F complex, which recognizes the 7-methylguanosine cap structure at the 5' end of messenger RNAs. The encoded protein aids in translation initiation by recruiting ribosomes to the 5'-cap structure. Association of this protein with the 4F complex is the rate-limiting step in translation initiation. This gene acts as a proto-oncogene, and its expression and activation is associated with transformation and tumorigenesis. Several pseudogenes of this gene are found on other chromosomes. Alternative splicing results in multiple transcript variants.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Polyclonal Antibodies

BiowMW :25KDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :AUTS19;CBP;EIF4E1;EIF4EL1;EIF4F;eIF-4E;EIF4E

Immunogen :A synthetic peptide of human eIF4E(NP_001959.1).

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of various lysates, using eIF4E antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 45s.

Western blot analysis of extracts from wild type and eIF4E knockout 293T cells, using eIF4E antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 45s.

Immunohistochemistry of paraffin-embedded human lung cancer using eIF4E antibody at dilution of 1:100 .Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Immunohistochemistry of paraffin-embedded human stomach using eIF4E antibody at dilution of 1:100 .Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Overexpression of hnRNPC2 induces multinucleation by repression of Aurora B in hepatocellular carcinoma cells

JournalOncology Letters

ApplicationIF

IF2.967

SpeciesHuamn

PMID23599772
3,3′-Diindolylmethane Protects against Cardiac Hypertrophy via 5′-Adenosine Monophosphate-Activated Protein Kinase-α2

JournalPLoS One

ApplicationWB

IF2.74

SpeciesMouse

PMID
Comparative proteomic profiling and possible toxicological mechanism of acute injury induced by carbon ion radiation in pubertal mice testes

JournalReproductive Toxicology

ApplicationWB,IF

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SpeciesMouse

PMID26257270