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Decorin polyclonal antibody

货号: BS6582

价格：￥ 1140

货期：现货（1-2个工作日）

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详细信息检测数据实验指南文献

No. :BS6582

Product Name :Decorin polyclonal antibody

Swiss-Prot :P07585

Host :Rabbit

Reactivity :Human, Mouse, Rat

Applications :WB, IHC, IF/ICC

Application_all :WB,1:500 - 1:2000|IHC,1:50 - 1:200|IF/ICC,1:50 - 1:200

Background :This gene encodes a member of the small leucine-rich proteoglycan family of proteins. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed to generate the mature protein. This protein plays a role in collagen fibril assembly. Binding of this protein to multiple cell surface receptors mediates its role in tumor suppression, including a stimulatory effect on autophagy and inflammation and an inhibitory effect on angiogenesis and tumorigenesis. This gene and the related gene biglycan are thought to be the result of a gene duplication. Mutations in this gene are associated with congenital stromal corneal dystrophy in human patients.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Polyclonal Antibodies

BiowMW :45KDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :DCN;CSCD;DSPG2;PG40;PGII;PGS2;SLRR1B;decorin;Decorin

Immunogen :Recombinant fusion protein of human Decorin(NP_001911.1).

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of extracts of HeLa cells, using Decorin antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 60s.

Western blot analysis of extracts of Mouse heart, using Decorin antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 180s.

Immunohistochemistry of paraffin-embedded human breast cancer using Decorin Rabbit pAb at dilution of 1:50 .Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Immunohistochemistry of paraffin-embedded human colon using Decorin Rabbit pAb at dilution of 1:50 .Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Expression of decorin in intestinal tissues of mice with inflammatory bowel disease and its correlation with autophagy

JournalExperimental and Therapeutic Medicine

ApplicationIHC,WB

IF1.785

SpeciesMouse

PMID
Decorin protects human hepatoma HepG2 cells against oxygen-glucose deprivation via modulating autophagy

JournalInternational Journal of Clinical and Experimental Medicine

ApplicationWB

IF0.166

SpeciesHuman

PMID26550264
Time-specific effects of acute eccentric exercise on myostatin, follistatin and decorin in the circulation and skeletal muscle in rats

JournalPHYSIOLOGICAL RESEARCH

ApplicationWB

IF2.139

SpeciesRat

PMID36281727