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Aromatase (CYP19A1) polyclonal antibody

货号: BS6580

价格：￥ 1140

货期：现货（1-2个工作日）

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说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :BS6580

Product Name :Aromatase (CYP19A1) polyclonal antibody

Swiss-Prot :P11511

Host :Rabbit

Reactivity :Human, Rat

Applications :WB, IHC, IF/ICC

Application_all :WB,1:1000 - 1:2000|IHC,1:50 - 1:200|IF/ICC,1:50 - 1:200

Background :This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and catalyzes the last steps of estrogen biosynthesis. Mutations in this gene can result in either increased or decreased aromatase activity; the associated phenotypes suggest that estrogen functions both as a sex steroid hormone and in growth or differentiation. Alternative promoter use and alternative splicing results in multiple transcript variants that have different tissue specificities.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Polyclonal Antibodies

BiowMW :48kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :CYP19A1;ARO;ARO1;CPV1;CYAR;CYP19;CYPXIX;P-450AROM;aromatase;Aromatase

Immunogen :Recombinant fusion protein of human Aromatase (CYP19A1)(NP_000094.2).

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of extracts of various cell lines, using Aromatase antibody at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 20s.

Immunohistochemistry of paraffin-embedded human placenta using Aromatase antibody at dilution of 1:100 .Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Immunohistochemistry of paraffin-embedded rat ovary using Aromatase antibody at dilution of 1:100 .Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Immunofluorescence analysis of HeLa cells using Aromatase antibody at dilution of 1:100 . Blue: DAPI for nuclear staining.

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PMID
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JournalMolecular Nutrition & Food Research

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SpeciesRat

PMID32022472