产品类别

联系我们

电话:025-68037686

地址:江苏生命科技创新园F6幢1层

订购:nanjing03@biogot.com

服务:biorase01@biogot.com

合作:lvyun@biogot.com

支持:may@biogot.com

CYP11A1 polyclonal antibody

货号: BS6578

价格：￥ 1140

货期：现货（1-2个工作日）

25ul 50ul 100ul 200ul

抗体定制服务咨询

说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :BS6578

Product Name :CYP11A1 polyclonal antibody

Swiss-Prot :P05108

Host :Rabbit

Reactivity :Human, Mouse, Rat

Applications :WB, IHC

Application_all :WB,1:500 - 1:1000|IHC,1:50 - 1:100

Background :This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the mitochondrial inner membrane and catalyzes the conversion of cholesterol to pregnenolone, the first and rate-limiting step in the synthesis of the steroid hormones. Two transcript variants encoding different isoforms have been found for this gene. The cellular location of the smaller isoform is unclear since it lacks the mitochondrial-targeting transit peptide.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Polyclonal Antibodies

BiowMW :50KDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :CYP11A1;CYP11A;CYPXIA1;P450SCC

Immunogen :A synthetic peptide of human CYP11A1(NP_000772.2).

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of extracts of Mouse testis, using CYP11A1 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 5s.

Western blot analysis of extracts of Rat testis, using CYP11A1 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 18s.

Immunohistochemistry of paraffin-embedded rat lung using CYP11A1 antibody at dilution of 1:100 .Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Immunohistochemistry of paraffin-embedded mouse liver using CYP11A1 antibody at dilution of 1:100 .Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Brain‐derived neurotrophic factor: A steroidogenic regulator of Leydig cells

JournalJournal Of Cellular Physiology

ApplicationWB

IF4.522

SpeciesMouse

PMID30628054
Discovery of methylpyrimidine ring-fused diterpenoid analogs as a novel testosterone synthesis promoter

JournalRSC Advances

ApplicationWB

IF3.119

SpeciesMouse

PMID
Moderate Aerobic Exercise Regulates Follicular Dysfunction by Initiating Brain-Derived Neurotrophic Factor (BDNF)-Mediated Anti-Apoptotic Signaling Pathways in Polycystic Ovary Syndrome

JournalJournal of Clinical Medicine

ApplicationWB

IF4.964

SpeciesRat

PMID36233452