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CYP2E1 polyclonal antibody

货号: BS6577

价格：￥ 1140

货期：现货（1-2个工作日）

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No. :BS6577

Product Name :CYP2E1 polyclonal antibody

Swiss-Prot :P05181

Host :Rabbit

Reactivity :Human,Mouse,Rat

Applications :WB, IHC, IF/ICC

Application_all :WB,1:500 - 1:2000|IHC,1:50 - 1:200|IF/ICC,1:50 - 1:200

Background :This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and is induced by ethanol, the diabetic state, and starvation. The enzyme metabolizes both endogenous substrates, such as ethanol, acetone, and acetal, as well as exogenous substrates including benzene, carbon tetrachloride, ethylene glycol, and nitrosamines which are premutagens found in cigarette smoke. Due to its many substrates, this enzyme may be involved in such varied processes as gluconeogenesis, hepatic cirrhosis, diabetes, and cancer.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Polyclonal Antibodies

BiowMW :55KDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :CYP2E1;CPE1;CYP2E;P450-J;P450C2E

Immunogen :Recombinant fusion protein of human CYP2E1(NP_000764.1).

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of extracts of Rat liver, using CYP2E1 Rabbit pAb antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 0.5s.

Immunohistochemistry of paraffin-embedded human liver cancer using CYP2E1 antibody at dilution of 1:100 .Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Immunohistochemistry of paraffin-embedded human stomach using CYP2E1 antibody at dilution of 1:100 .Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Liver cancer-related gene CYP2E1 expression in HBV transgenic mice with acute liver injury

JournalTumor Biology

ApplicationIHC

IF0

SpeciesMouse

PMID24318994
Demethyleneberberine, a Natural Mitochondria-Targeted Antioxidant, Inhibits Mitochondrial Dysfunction, Oxidative Stress, and Steatosis in Alcoholic Liver Disease Mouse Model

JournalJournal Of Pharmacology And Experimental Therapeutics

ApplicationIHC

IF4.03

SpeciesMouse

PMID25362106
Moringa oleifera mitigates ethanol-induced oxidative stress, fatty degeneration and hepatic steatosis by promoting Nrf2 in mice

JournalPhytomedicine

ApplicationIHC,WB

IF5.34

SpeciesMouse

PMID35358929