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CYP2E1 polyclonal antibody

货号: BS6577
价格 1140
货期 现货(1-2个工作日)
25ul 50ul 100ul 200ul
No. :BS6577
Product Name :CYP2E1 polyclonal antibody
Swiss-Prot :P05181
Host :Rabbit
Reactivity :Human,Mouse,Rat
Applications :WB, IHC, IF/ICC
Application_all :WB,1:500 - 1:2000|IHC,1:50 - 1:200|IF/ICC,1:50 - 1:200
Background :This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and is induced by ethanol, the diabetic state, and starvation. The enzyme metabolizes both endogenous substrates, such as ethanol, acetone, and acetal, as well as exogenous substrates including benzene, carbon tetrachloride, ethylene glycol, and nitrosamines which are premutagens found in cigarette smoke. Due to its many substrates, this enzyme may be involved in such varied processes as gluconeogenesis, hepatic cirrhosis, diabetes, and cancer.
Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Specificity :Polyclonal Antibodies
BiowMW :55KDa
Note :For research use only, not for use in diagnostic procedure.
Alternative Name :CYP2E1;CPE1;CYP2E;P450-J;P450C2E
Immunogen :Recombinant fusion protein of human CYP2E1(NP_000764.1).
Conjugate :Unconjugated
Modification :Unmodification
Western blot analysis of extracts of Rat liver, using CYP2E1 Rabbit pAb antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 0.5s.
Immunohistochemistry of paraffin-embedded human liver cancer using CYP2E1 antibody at dilution of 1:100 .Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunohistochemistry of paraffin-embedded human stomach using CYP2E1 antibody at dilution of 1:100 .Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
  • Liver cancer-related gene CYP2E1 expression in HBV transgenic mice with acute liver injury
    JournalTumor Biology
    ApplicationIHC
    IF0
    SpeciesMouse
  • Demethyleneberberine, a Natural Mitochondria-Targeted Antioxidant, Inhibits Mitochondrial Dysfunction, Oxidative Stress, and Steatosis in Alcoholic Liver Disease Mouse Model
    JournalJournal Of Pharmacology And Experimental Therapeutics
    ApplicationIHC
    IF4.03
    SpeciesMouse
  • Moringa oleifera mitigates ethanol-induced oxidative stress, fatty degeneration and hepatic steatosis by promoting Nrf2 in mice
    JournalPhytomedicine
    ApplicationIHC,WB
    IF5.34
    SpeciesMouse

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400-660-9091

电话:025-68037686

地址:江苏生命科技创新园F6幢1层

订购:nanjing03@biogot.com

服务:biorase01@biogot.com

合作:lvyun@biogot.com

支持:may@biogot.com