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CSNK2A2 polyclonal antibody

货号: BS6571

价格：￥ 1140

货期：现货（1-2个工作日）

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No. :BS6571

Product Name :CSNK2A2 polyclonal antibody

Swiss-Prot :P19784

Host :Rabbit

Reactivity :Human, Mouse, Rat

Applications :WB, IHC, IF/ICC

Application_all :WB,1:500 - 1:2000|IHC,1:50 - 1:200|IF/ICC,1:50 - 1:200

Background :Catalytic subunit of a constitutively active serine/threonine-protein kinase complex that phosphorylates a large number of substrates containing acidic residues C-terminal to the phosphorylated serine or threonine. Regulates numerous cellular processes, such as cell cycle progression, apoptosis and transcription, as well as viral infection. May act as a regulatory node which integrates and coordinates numerous signals leading to an appropriate cellular response. During mitosis, functions as a component of the p53/TP53-dependent spindle assembly checkpoint (SAC that maintains cyclin-B-CDK1 activity and G2 arrest in response to spindle damage. Also required for p53/TP53-mediated apoptosis, phosphorylating 'Ser-392' of p53/TP53 following UV irradiation.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Polyclonal Antibodies

BiowMW :40KDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :CSNK2A2;CK2A2;CK2alpha;CSNK2A1

Immunogen :A synthetic peptide of human CSNK2A2(NP_001887.1).

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of extracts of various cell lines, using CSNK2A2 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 30s.

Western blot analysis of extracts of HeLa cells, using CSNK2A2 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 180s.

Immunofluorescence analysis of NIH/3T3 cells using CSNK2A2 Rabbit pAb at dilution of 1:100 . Blue: DAPI for nuclear staining.

Immunofluorescence analysis of PC-12 cells using CSNK2A2 Rabbit pAb at dilution of 1:100 . Blue: DAPI for nuclear staining.

Ablation of beta subunit of protein kinase CK2 in mouse oocytes causes follicle atresia and premature ovarian failure

JournalCell Death & Disease

ApplicationWB

IF6.304

SpeciesMouse

PMID29725001
Deletion of Ck2β gene causes germ cell development arrest and azoospermia in male mice

JournalCell Proliferation

ApplicationWB

IF5.753

SpeciesMouse

PMID31755150