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ZNF318 Polyclonal Antibody

货号: BS65684

价格：￥ ——

货期：现货（1-2个工作日）

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说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :BS65684

Product Name :ZNF318 Polyclonal Antibody

Swiss-Prot :Q5VUA4

Host :Rabbit

Reactivity :Human,Mouse,Rat,Dog,Pig,Horse,Rabbit,

Applications :WB,IHC-P,

Application_all :WB=1:500-2000 IHC-P=1:100-500

Background :ZNF318 is a 2279 amino acid endocrine regulatory protein that localizes to the nucleus. Highly expressed in testis, ovaries and kidneys, ZNF318 is a co-repressor of androgen receptor (AR)-mediated transcriptional activation and is thought to regulate transcription during spermatogenesis. ZNF318 interacts with the N-terminal domain of AR and contains two matrin-type zinc fingers. Two isoforms of ZNF318, designated TZF and TZF-L, are produced due to alternative splicing events. Each of these splice variants are thought to have unique roles in transcriptional regulation. While the TZF isoform functions as a repressor of AR-mediated transcriptional activation, the TZF-L isoform is thought to enhance AR-mediated transcriptional activation.

Product :0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

Purification&Purity :affinity purified by Protein A

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :ZNF318 Polyclonal Antibody detects endogenous levels of ZNF318 protein.

BiowMW :125 kD

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :Endocrine Regulatory Protein; HGNC13578; HRIHFB2436; ZFP318; Zinc Finger Protein 318; ZN318_HUMAN.

Immunogen :KLH conjugated synthetic peptide derived from human ZNF318

Conjugate :Unconjugated

Modification :Unmodification

Sample: Lymph node(Mouse) Lysate at 40 ug Testis (Mouse) Lysate at 40 ug A549(Human) Cell Lysate at 30 ug HL60(Human) Cell Lysate at 30 ug 293T(Human) Cell Lysate at 30 ug Primary: Anti- ZNF318 at 1/1000 dilution

Paraformaldehyde-fixed, paraffin embedded (rat uterus); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (ZNF318) Polyclonal Antibody, Unconjugated (bs-11414R) at 1:200 overnight at 4°C