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ERK1 + ERK2 Polyclonal Antibody

货号: BS65610

价格：￥ 1140

货期：现货（1-2个工作日）

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说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :BS65610

Product Name :ERK1 + ERK2 Polyclonal Antibody

Swiss-Prot :P63085

Host :Rabbit

Reactivity :Human, Mouse, Rat, (predicted: Chicken, Dog, Pig, Cow, Horse, Rabbit, )

Applications :WB，ICC，

Application_all :WB=1:500-2000 ICC=1:100

Background :The protein encoded by this gene is a member of the MAPkinase family. MAP kinases, also known as extracellularsignal-regulated kinases (ERKs), act in a signaling cascade thatregulates various cellular processes such as proliferation,differentiation, and cell cycle progression in response to avariety of extracellular signals. This kinase is activated byupstream kinases, resulting in its translocation to the nucleuswhere it phosphorylates nuclear targets. Alternatively splicedtranscript variants encoding different protein isoforms have beendescribed. [provided by RefSeq, Jul 2008].

Product :0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

Purification&Purity :affinity purified by Protein A

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :ERK1 + ERK2 Polyclonal Antibody detects endogenous levels of ERK1 + ERK2 protein.

BiowMW :42/44kD

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :ERK1/2; ERK1+ERK2; ERK1/ERK2; ERK 1; ERK 2; ERK-2; ERK1; ERK2; ERT1; ERT2; Extracellular signal regulated kinase 1; Extracellular signal regulated kinase 2; Extracellular signal-regulated kinase 2; HS44KDAP; HUMKER1A; Insulin stimulated MAP2 kinase; MAP kinase 1; MAP kinase 2; MAP kinase isoform p42; MAP kinase isoform p44; MAPK 1; MAPK 2; MAPK 3; MAPK1; MAPK2; MAPK3; MGC20180; Microtubule associated protein 2 kinase; Mitogen activated protein kinase 1; Mitogen activated protein kinase 2; Mitogen activated protein kinase 3; Mitogen-activated protein kinase 1; Mitogen-activated protein kinase 2; MK03_HUMAN; MK01_HUMAN; p38; p40; p41; p41mapk; p42 MAPK; p42-MAPK; p42MAPK; p44 ERK1; p44 MAPK; p44ERK1; p44MAPK; PRKM 1; PRKM 2; PRKM 3; PRKM1; PRKM2; PRKM3; Protein kinase mitogen activated 1; Protein kinase mitogen activated 2; Protein kinase mitogen activated 3; Protein tyrosine kinase ERK 2.

Immunogen :KLH conjugated synthetic peptide derived from human ERK1/2:251-358/358

Conjugate :Unconjugated

Modification :Unmodification

Sample: A431(Human) Cell Lysate at 30 ug Primary: Anti-ERK1 + ERK2 at 1/1000 dilution

Sample: A549 Cell Lysate at 30 ug Primary: Anti- ERK1+ERK2 at 1/300 dilution

Tissue/cell: HUVEC cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (ERK1 + ERK2) Polyclonal Antibody, Unconjugated 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue,) was used to stain the cell nuclei.

Synergistic Effect and Mechanism of Apoptosis Induction by Morphine and the HIV-1gp120V3 Loop in Hippocampal Neurons

JournalJournal of Neuroimmune Pharmacology

ApplicationWB

IF4.147

SpeciesRat

PMID
Flower extract of Caragana sinica. ameliorates DSS-induced ulcerative colitis by affecting TLR4/NF-κB and TLR4/MAPK signaling pathway in a mouse model

JournalIranian Journal of Basic Medical Sciences

ApplicationWB

IF2.699

SpeciesMouse

PMID34249260