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BUB1B polyclonal antibody

货号: BS6539

价格：￥ 1140

货期：现货（1-2个工作日）

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说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :BS6539

Product Name :BUB1B polyclonal antibody

Swiss-Prot :O60566

Host :Rabbit

Reactivity :Human, Mouse, Rat

Applications :WB, IF/ICC

Application_all :WB,1:500 - 1:2000|IF/ICC,1:50 - 1:200

Background :This gene encodes a kinase involved in spindle checkpoint function. The protein has been localized to the kinetochore and plays a role in the inhibition of the anaphase-promoting complex/cyclosome (APC/C), delaying the onset of anaphase and ensuring proper chromosome segregation. Impaired spindle checkpoint function has been found in many forms of cancer.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Polyclonal Antibodies

BiowMW :55kDa/120kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :BUB1B;BUB1beta;BUBR1;Bub1A;MAD3L;MVA1;SSK1;hBUBR1

Immunogen :Recombinant fusion protein of human BUB1B(NP_001202.4).

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of extracts of various cell lines, using BUB1B antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

Immunofluorescence analysis of NIH-3T3 cells using BUB1B antibody at dilution of 1:100. Blue: DAPI for nuclear staining.