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SIRT1 polyclonal antibody

货号: BS6494

价格：￥ 1140

货期：现货（1-2个工作日）

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详细信息检测数据实验指南文献

No. :BS6494

Product Name :SIRT1 polyclonal antibody

Swiss-Prot :Q96EB6

Host :Rabbit

Reactivity :Human, Mouse

Applications :WB, IHC

Application_all :WB,1:500 - 1:2000|IHC,1:50 - 1:200

Background :This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class I of the sirtuin family. Alternative splicing results in multiple transcript variants.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Polyclonal Antibodies

BiowMW :120KDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :SIR2;SIR2L1;SIR2alpha;SIRT1;sirtuin 1

Immunogen :A synthetic peptide of human SIRT1(NP_036370.2).

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of various lysates, using SIRT1 antibody at 1:2950 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 30s.

Immunohistochemistry of paraffin-embedded human colon using [KO Validated] SIRT1 Rabbit pAb at dilution of 1:200 .Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

HucMSC exosome-delivered 14-3-3ζ alleviates ultraviolet radiation-induced photodamage via SIRT1 pathway modulation

JournalAging-US

ApplicationWB,IF

IF4.831

SpeciesRat,Human

PMID33882455
Smooth Muscle Sirtuin 1 Blocks Thoracic Aortic Aneurysm/Dissection Development in Mice

JournalCardiovascular Drugs And Therapy

ApplicationWB

IF4.069

SpeciesMouse,Rat

PMID32564302
Paeonol Ameliorates Diabetic Renal Fibrosis Through Promoting the Activation of the Nrf2/ARE Pathway via Up-Regulating Sirt1

JournalFrontiers in Pharmacology

ApplicationWB

IF3.845

SpeciesRat,Mouse

PMID29867511
MicroRNA-128 is involved in dexamethasone-induced lipid accumulation via repressing SIRT1 expression in cultured pig preadipocytes

JournalJournal Of Steroid Biochemistry And Molecular Biology

ApplicationWB

IF3.785

SpeciesPig

PMID30394333
Protective effect of small peptides from Periplaneta americana on cyclophosphamide-induced premature ovarian failure

JournalJournal Of Obstetrics And Gynaecology Research

ApplicationIHC

IF1.73

SpeciesMouse

PMID34729871