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MCL1 polyclonal antibody

货号: BS6490

价格：￥ 1140

货期：现货（1-2个工作日）

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详细信息检测数据实验指南文献

No. :BS6490

Product Name :MCL1 polyclonal antibody

Swiss-Prot :Q07820

Host :Rabbit

Reactivity :Human, Mouse

Applications :WB, IHC, IF/ICC

Application_all :WB,1:500 - 1:2000|IHC,1:100 - 1:200|IF/ICC,1:50 - 1:200

Background :This gene encodes an anti-apoptotic protein, which is a member of the Bcl-2 family. Alternative splicing results in multiple transcript variants. The longest gene product (isoform 1) enhances cell survival by inhibiting apoptosis while the alternatively spliced shorter gene products (isoform 2 and isoform 3) promote apoptosis and are death-inducing.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Polyclonal Antibodies

BiowMW :37kDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :MCL1;BCL2L3;EAT;MCL1-ES;MCL1L;MCL1S;Mcl-1;TM;bcl2-L-3;mcl1/EAT;MCL1

Immunogen :A synthetic peptide of human MCL1(NP_068779.1).

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of extracts of various cell lines, using MCL1 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 30s.

Immunohistochemistry of paraffin-embedded mouse brain using MCL1 Rabbit pAb at dilution of 1:100 .Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Immunofluorescence analysis of NIH/3T3 cells using MCL1 Rabbit pAb at dilution of 1:100 . Blue: DAPI for nuclear staining.

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