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[KO Validated] CD133 polyclonal antibody

货号: BS6480

价格：￥ 1140

货期：现货（1-2个工作日）

25ul 50ul 100ul 200ul

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说明书 MSDS SHIP

详细信息检测数据实验指南文献

No. :BS6480

Product Name :[KO Validated] CD133 polyclonal antibody

Swiss-Prot :O43490

Host :Rabbit

Reactivity :Human, Mouse, Rat

Applications :WB, IF/ICC, IP

Application_all :WB,1:1000 - 1:3000|IF/ICC,1:50 - 1:200|IP,1:50 - 1:200

Background :This gene encodes a pentaspan transmembrane glycoprotein. The protein localizes to membrane protrusions and is often expressed on adult stem cells, where it is thought to function in maintaining stem cell properties by suppressing differentiation. Mutations in this gene have been shown to result in retinitis pigmentosa and Stargardt disease. Expression of this gene is also associated with several types of cancer. This gene is expressed from at least five alternative promoters that are expressed in a tissue-dependent manner. Multiple transcript variants encoding different isoforms have been found for this gene.

Product :1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification&Purity :The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage&Stability :Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity :Polyclonal Antibodies

BiowMW :120KDa

Note :For research use only, not for use in diagnostic procedure.

Alternative Name :PROM1;AC133;CD133;CORD12;MCDR2;MSTP061;PROML1;RP41;STGD4

Immunogen :Recombinant fusion protein of human CD133(NP_006008.1).

Conjugate :Unconjugated

Modification :Unmodification

Western blot analysis of extracts of HCT116 cells, using CD133 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 30s.

Western blot analysis of extracts from normal and CD133 Rabbit pAb knockout HCT116 cells, using CD133 Rabbit pAb antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 30s.

Immunoprecipitation analysis of 200ug extracts of HT-29 cells using 3ug CD133 antibody . Western blot was performed from the immunoprecipitate using CD133 antibody at a dilution of 1:1000.

EGCG inhibits properties of glioma stem-like cells and synergizes with temozolomide through downregulation of P-glycoprotein inhibition

JournalJournal Of Neuro-Oncology

ApplicationWB

IF3.267

SpeciesHuman

PMID25173233
Novel composite drug delivery system as a novel radio sensitizer for the local treatment of cervical carcinoma

JournalDrug Delivery

ApplicationIHC

IF6.419

SpeciesMouse

PMID28797171
Efficient inhibition of cervical cancer by dual drugs loaded in biodegradable thermosensitive hydrogel composites

JournalOncotarget

ApplicationIHC

IF0

SpeciesMouse

PMID29416613